Direct and indirect fluorescent detection of tetracyclines using dually emitting carbon dots
文献类型:期刊论文
作者 | Qu, Fei ; Sun, Zhe ; Liu, Dongya ; Zhao, Xianen ; You, Jinmao |
刊名 | MICROCHIMICA ACTA
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出版日期 | 2016-09-01 |
英文摘要 | The authors describe dual-emission carbon nanodots containing blue emitters (BE; peak emission at 385nm under 315 nm excitation) and yellow emitters (YE; peak emission at 530 nm under 365nm excitation), and how they can be applied to direct and indirect determination of tetracyclines (TCs). The direct detection scheme is based on the finding that tetracycline (TET), oxytetracycline, chlortetracycline and doxycycline quench the two emissions of the carbon dots. While direct determination is rapid and convenient, it cannot differentiate between TCs. The indirect detection scheme, in contrast, is based on the finding that Al (III) ions enhance the fluorescence of the YE in the carbon dots, and that they cause a blue shift in emission. It is, however, known that TET forms a strong complex with Al (III), and this can inhibit the interaction between Al (III) and the YE, so that the fluorescence of YE is not enhanced and blue-shifted by Al (III) in the presence of TET. This finding is exploited in a fluorescence turn-on/off assay for TET that can distinguish TET from other TCs. The linear range of indirect determination for TET extends from 1 nM to 30 mu M, and the limit of detection is 0.52 nM. The indirect method was successfully applied to the determination of TET in spiked milk, fish and pork, and recoveries ranged from 91.7 to 102 %. |
源URL | [http://ir.nwipb.ac.cn/handle/363003/6428] ![]() |
专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Qu, Fei,Sun, Zhe,Liu, Dongya,et al. Direct and indirect fluorescent detection of tetracyclines using dually emitting carbon dots[J]. MICROCHIMICA ACTA,2016. |
APA | Qu, Fei,Sun, Zhe,Liu, Dongya,Zhao, Xianen,&You, Jinmao.(2016).Direct and indirect fluorescent detection of tetracyclines using dually emitting carbon dots.MICROCHIMICA ACTA. |
MLA | Qu, Fei,et al."Direct and indirect fluorescent detection of tetracyclines using dually emitting carbon dots".MICROCHIMICA ACTA (2016). |
入库方式: OAI收割
来源:西北高原生物研究所
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