Extraction and Separation of Vitisin D, Ampelopsin B and cis-Vitisin A from Iris lactea Pall. var. chinensis (Fisch.) Koidz by Alkaline Extraction-Acid Precipitation and High-Speed Counter-Current Chromatography
文献类型：期刊论文
| 作者 | Lv, Huanhuan ; Zhou, Wenna ; Wang, Xiaoyan ; Wang, Zhenhua ; Suo, Yourui ; Wang, Honglun |
| 刊名 | JOURNAL OF CHROMATOGRAPHIC SCIENCE
 |
| 出版日期 | 2016-05-01 |
| 英文摘要 | Naturally occurring oligostilbenes are receiving more attention because they exhibit several beneficial effects for health, including hepatoprotective, antitumor, anti-adipogenic, antioxidant, antiaging, anti-inflammatory, anti-microbial, antiviral, immunosuppressive and neuroprotective activities. Thus, they could be of some potentially therapeutic values for several diseases. In this study, we adopted the alkaline extraction-acid precipitation (AEAP) method for extraction of oligostilbenes from the seed kernel of Iris lactea. Then, the high-speed counter-current chromatography (HSCCC) was used for preparative isolation and purification of oligostilbenes from the AEAP extracts. Finally, three oligostilbenes, namely vitisin D (73 mg), ampelopsin B (25 mg) and cis-vitisin A (16 mg), were successfully fractionated by HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (2:5:3:6, v/v/v/v) from 300 mg of the AEAP extracts in similar to 190 min. The purities of the three isolated oligostilbenes were all over 95.0% as analyzed by high performance liquid chromatography. They all were isolated from I. lactea for the first time. The method of AEAP for the preparation of the oligostilbene-enriched crude sample was simple, and the HSCCC technique for the isolation and purification of oligostilbenes was efficient. |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6442]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Lv, Huanhuan,Zhou, Wenna,Wang, Xiaoyan,et al. Extraction and Separation of Vitisin D, Ampelopsin B and cis-Vitisin A from Iris lactea Pall. var. chinensis (Fisch.) Koidz by Alkaline Extraction-Acid Precipitation and High-Speed Counter-Current Chromatography[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2016. |
| APA | Lv, Huanhuan,Zhou, Wenna,Wang, Xiaoyan,Wang, Zhenhua,Suo, Yourui,&Wang, Honglun.(2016).Extraction and Separation of Vitisin D, Ampelopsin B and cis-Vitisin A from Iris lactea Pall. var. chinensis (Fisch.) Koidz by Alkaline Extraction-Acid Precipitation and High-Speed Counter-Current Chromatography.JOURNAL OF CHROMATOGRAPHIC SCIENCE. |
| MLA | Lv, Huanhuan,et al."Extraction and Separation of Vitisin D, Ampelopsin B and cis-Vitisin A from Iris lactea Pall. var. chinensis (Fisch.) Koidz by Alkaline Extraction-Acid Precipitation and High-Speed Counter-Current Chromatography".JOURNAL OF CHROMATOGRAPHIC SCIENCE (2016). |
入库方式： OAI收割
来源：西北高原生物研究所
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