Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization
文献类型：期刊论文
| 作者 | Lv, Tao ; Zhao, Xian-En ; Zhu, Shuyun ; Ji, Zhongyin ; Chen, Guang ; Sun, Zhiwei ; Song, Cuihua ; You, Jinmao ; Suo, Yourui ; Zhao, XE (reprint author), Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China. |
| 刊名 | CHROMATOGRAPHIA
 |
| 出版日期 | 2014-12-01 |
| 英文摘要 | A novel hyphenated technique based on ultrasonic-assisted dispersive liquid-liquid microextraction (UA-DLLME) coupled with derivatization has been established for the determination of brassinolide (BL, a representative of brassinosteroids) by HPLC fluorescence detection. 9-Phenanthreneboronic acid is used as labeling reagent of BL. UA-DLLME parameters containing type and volume of extraction and disperser solvent, pH and ultrasonication time are optimized. Derivatization parameters are optimized included amount of 9-phenanthreneboronic acid, volume ratio of pyridine, derivatization time and temperature. Under optimal conditions, quantitative linear range of BL is 50-1,000 ng L-1 and excellent linear response is observed with correlation coefficient of 0.9996. Limit of detection and limit of quantification are calculated as 8.0 and 25.0 ng L-1, respectively. RSDs of retention time and peak area are in the range of 0.68-0.97 % and 4.61-6.54 % for intra-day precision, 1.32-1.94 % and 7.28-9.75 % for inter-day precision, respectively. Accuracy is satisfactory in the range of 82.3-125.1 %. RSDs' values of repeatability are in the range of 0.82-1.79 and 3.95-8.53 % for retention time and peak area, respectively. Enrichment factor for BL is 189. The results of recovery and matrix effect are in the range of 82.0-108.6 and 90.0-115.3 %, respectively. The proposed method has been applied for the determination of BL in Arabidopsis thaliana, Daucus carota and Brassica campestris L. leaves with much higher sensitivity than many other methods. |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6589]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Lv, Tao,Zhao, Xian-En,Zhu, Shuyun,et al. Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization[J]. CHROMATOGRAPHIA,2014. |
| APA | Lv, Tao.,Zhao, Xian-En.,Zhu, Shuyun.,Ji, Zhongyin.,Chen, Guang.,...&Zhao, XE .(2014).Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization.CHROMATOGRAPHIA. |
| MLA | Lv, Tao,et al."Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization".CHROMATOGRAPHIA (2014). |
入库方式： OAI收割
来源：西北高原生物研究所
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