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Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology

文献类型：期刊论文

作者Liu, Zenggen2,3,4; Tao, Yanduo2,3,4; Mei, Lijuan2,3,4; Dang, Jun2,3,4; Shen, Na1,2,3,4; Cui, Yulei1,2,3,4
刊名JOURNAL OF SEPARATION SCIENCE
出版日期2017-07-01
卷号40期号:14页码:2895-2905
关键词Arenaria Kansuensis Bioactive Equivalence Of Combinatorial Components Liquid-liquid Extraction Solid-phase Extraction Two-dimensional Chromatography
ISSN号1615-9306
DOI10.1002/jssc.201700144
文献子类Article
英文摘要Bioactive equivalent combinatorial components play a critical role in herbal medicines. However, how to discover and enrich them efficiently is a question for herbal pharmaceuticals researchers. In our work, a novel two-dimensional reversed-phase/hydrophilic interaction high-performance liquid chromatography method was established to perform real-time components trapping and combining for preparation and isolation of coeluting components. Arenaria kansuensis was taken as an example, and solid-phase extraction coupled with liquid-liquid extraction as a simple and efficient method for enriching trace components, reversed phase column coupled with hydrophilic interaction liquid chromatography XAmide column as two-dimensional chromatography technology for isolation and preparation of coeluting constituents, enzyme-linked immune-sorbent assay as bio-guided assay, and anti-inflammatory bioactivity evaluation for bioactive constituents. A combination of 12 beta-carboline alkaloidswas identified as anti-inflammatory bioactive equivalent combinatorial components from A. kansuensis, which accounts for 1.9% w/w of original A. kansuensis. This work answers the key question of which are real anti-inflammatory components from A. kansuensis and provides a fast and efficient approach for discovering and enriching trace beta-carboline alkaloids from herbal medicines for the first time. More importantly, the discovery of bioactive equivalent combinatorial components could improve the quality control of herbal products and inspire a herbal medicine based on combinatorial therapeutics.
WOS关键词PICRASMA-QUASSIOIDES ; MEDICINE
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000406244500004
出版者WILEY-V C H VERLAG GMBH
资助机构West Light Foundation of The Chinese Academy of Sciences for Doctors ; Natural Science Foundation of Qinghai Province(2015-ZJ-927Q) ; Science and Technology Major Project of Qinghai Province, China(2014-GX-A3A) ; Strategic network planning project of biological resources service from Chinese academy of science(ZSTH-027) ; Significant Science & Technological Project of Qinghai Province(2014-GX-A3A-01)
源URL[http://ir.nwipb.ac.cn/handle/363003/9794]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
通讯作者Liu, Zenggen; Tao, Yanduo
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Key Lab Tibetan Med Res Qinghai Prov, Xining, Qinghai, Peoples R China
3.Chinese Acad Sci, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China
4.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Qinghai, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Zenggen,Tao, Yanduo,Mei, Lijuan,et al. Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(14):2895-2905.
APA Liu, Zenggen,Tao, Yanduo,Mei, Lijuan,Dang, Jun,Shen, Na,&Cui, Yulei.(2017).Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology.JOURNAL OF SEPARATION SCIENCE,40(14),2895-2905.
MLA Liu, Zenggen,et al."Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology".JOURNAL OF SEPARATION SCIENCE 40.14(2017):2895-2905.

入库方式： OAI收割

来源：西北高原生物研究所

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