Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection
文献类型：期刊论文
| 作者 | You, Jinmao ; Zhu, Fang ; Zhao, Wenchen ; Zhao, Xian'en ; Suo, Yourui ; Liu, Sujuan |
| 刊名 | EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
 ; You Jinmao,Zhu Fang,Zhao Wenchen,Zhao Xian'en,Suo Yourui,Liu Sujuan.Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,2007,109(3):225-236
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| 出版日期 | 2007-03-01 |
| 英文摘要 | A sensitive method for the determination of free fatty acids using 2-(2-(anthracen-10-yl)-1H-naphtho[2,3-dimidazol-1-yl) ethyl-p-toluenesuIfonate (ANITS) as tagging reagent with fluorescence detection has been developed. ANITS could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 degrees C for 40 min in N,N-dimethylformamide solvent. From the extracts of rape bee pollen samples, 20 free fatty acids were sensitively determined. Fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C8 column by HPLC in conjunction with gradient elution. The corresponding derivatives were identified by post-column APCI/MS in positive-ion detection mode. ANITS-fatty acid derivatives gave an intense molecular ion peak at mlz [M+H](+); with MS/MS analysis, the collision-induced dissociation spectra of m/z [M+H](+) produced the specific fragment ions at mlz [M-345](+) and mlz 345.0 (here, m/z 345 is the core structural moiety of the ANITS molecule). The fluorescence excitation and emission wavelengths of the derivatives were lambda(ex) = 250 nm and lambda(em) = 512 nm, respectively. Linear correlation coefficients for all fatty acid derivatives are > 0.9999. Detection limits, at a signal-to-noise ratio of 3 : 1, are 24.76-98.79 fmol for the labeled fatty acids.; A sensitive method for the determination of free fatty acids using 2-(2-(anthracen-10-yl)-1H-naphtho[2,3-dimidazol-1-yl) ethyl-p-toluenesuIfonate (ANITS) as tagging reagent with fluorescence detection has been developed. ANITS could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 degrees C for 40 min in N,N-dimethylformamide solvent. From the extracts of rape bee pollen samples, 20 free fatty acids were sensitively determined. Fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C8 column by HPLC in conjunction with gradient elution. The corresponding derivatives were identified by post-column APCI/MS in positive-ion detection mode. ANITS-fatty acid derivatives gave an intense molecular ion peak at mlz [M+H](+); with MS/MS analysis, the collision-induced dissociation spectra of m/z [M+H](+) produced the specific fragment ions at mlz [M-345](+) and mlz 345.0 (here, m/z 345 is the core structural moiety of the ANITS molecule). The fluorescence excitation and emission wavelengths of the derivatives were lambda(ex) = 250 nm and lambda(em) = 512 nm, respectively. Linear correlation coefficients for all fatty acid derivatives are > 0.9999. Detection limits, at a signal-to-noise ratio of 3 : 1, are 24.76-98.79 fmol for the labeled fatty acids. |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/1287]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | You, Jinmao,Zhu, Fang,Zhao, Wenchen,et al. Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection[J]. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, You Jinmao,Zhu Fang,Zhao Wenchen,Zhao Xian'en,Suo Yourui,Liu Sujuan.Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,2007,109(3):225-236,2007. |
| APA | You, Jinmao,Zhu, Fang,Zhao, Wenchen,Zhao, Xian'en,Suo, Yourui,&Liu, Sujuan.(2007).Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection.EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY. |
| MLA | You, Jinmao,et al."Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection".EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY (2007). |
入库方式： OAI收割
来源：西北高原生物研究所
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