Molecular characterization of LMW glutenin genes from Taeniatherum Nevski
文献类型：期刊论文
| 作者 | Han, Chang ; Yan, Ze-Hong ; Dai, Shou-Fen ; Liu, Deng-Cai ; Wei, Yu-Ming ; Zheng, You-Liang ; Lan, Xiu-Jin ; Peng, Yuan-Ying |
| 刊名 | GENETIC RESOURCES AND CROP EVOLUTION
 ; Han, C; Yan, ZH; Dai, SF; Liu, DC; Wei, YM; Zheng, YL; Lan, XJ; Peng, YY.Molecular characterization of LMW glutenin genes from Taeniatherum Nevski,GENETIC RESOURCES AND CROP EVOLUTION,2011,58(7):1029-1039
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| 出版日期 | 2011-10-01 |
| 英文摘要 | We characterized 45 LMW glutenin genes from three diploid species of Taeniatherum using 63 primer combinations, designed according to 264 genes reported in wheat and related species. The genes had 909-1,059 bp nucleotides and 301-351 amino acids. The deduced peptides shared similar structures with LMW-m proteins of wheat. The 45 genes shared 77.2-99.7% identities in peptide sequence among each other and 60.0-82.0% identities to proteins from wheat and related species. They were divided into five types according to the N-terminals, starting with METSCIP-, METSRVP-, METGRIP-, METGSIP- and VETSCIP-. The last three and some other structural domain variations were not reported previously in the Triticeae. Thirty-three genes encoded full mature proteins with intact ORFs, whereas the other 12 were pseudogenes with incomplete ORFs, in-frame stop codons or frame-shift mutations. Phylogenetic analysis showed that orthologous genes from Taeniatherum were more similar to those in the B and D genomes than in the A genome.; We characterized 45 LMW glutenin genes from three diploid species of Taeniatherum using 63 primer combinations, designed according to 264 genes reported in wheat and related species. The genes had 909-1,059 bp nucleotides and 301-351 amino acids. The deduced peptides shared similar structures with LMW-m proteins of wheat. The 45 genes shared 77.2-99.7% identities in peptide sequence among each other and 60.0-82.0% identities to proteins from wheat and related species. They were divided into five types according to the N-terminals, starting with METSCIP-, METSRVP-, METGRIP-, METGSIP- and VETSCIP-. The last three and some other structural domain variations were not reported previously in the Triticeae. Thirty-three genes encoded full mature proteins with intact ORFs, whereas the other 12 were pseudogenes with incomplete ORFs, in-frame stop codons or frame-shift mutations. Phylogenetic analysis showed that orthologous genes from Taeniatherum were more similar to those in the B and D genomes than in the A genome. |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1506]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Han, Chang,Yan, Ze-Hong,Dai, Shou-Fen,et al. Molecular characterization of LMW glutenin genes from Taeniatherum Nevski[J]. GENETIC RESOURCES AND CROP EVOLUTION, Han, C; Yan, ZH; Dai, SF; Liu, DC; Wei, YM; Zheng, YL; Lan, XJ; Peng, YY.Molecular characterization of LMW glutenin genes from Taeniatherum Nevski,GENETIC RESOURCES AND CROP EVOLUTION,2011,58(7):1029-1039,2011. |
| APA | Han, Chang.,Yan, Ze-Hong.,Dai, Shou-Fen.,Liu, Deng-Cai.,Wei, Yu-Ming.,...&Peng, Yuan-Ying.(2011).Molecular characterization of LMW glutenin genes from Taeniatherum Nevski.GENETIC RESOURCES AND CROP EVOLUTION. |
| MLA | Han, Chang,et al."Molecular characterization of LMW glutenin genes from Taeniatherum Nevski".GENETIC RESOURCES AND CROP EVOLUTION (2011). |
入库方式： OAI收割
来源：西北高原生物研究所
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