Development of a pair of differential H/D isotope-coded derivatization reagents d(0)/d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl) phenlamine and its application for determination of aldehydes in selected aquatic products by liquid chromatography-tandem mass spectrometry.
文献类型：期刊论文
| 作者 | Sun, Zhiwei ; Wang, Xiaoxiang ; Cai, Yiping ; Fu, Junqing ; You, Jinmao |
| 刊名 | TALANTA
 ; Sun, ZW; Wang, XX; Cai, YP; Fu, JQ; You, JM.Development of a pair of differential H/D isotope-coded derivatization reagents d(0)/d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl) phenlamine and its application for determination of aldehydes in selected aquatic products by liquid chromatography-tandem mass spectrometry.,TALANTA,2014,120():84
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| 出版日期 | 2014-03-01 |
| 英文摘要 | A new pair of derivatization reagents, d(0)-4-(1-methyl-1H-phenanthro[9,10-dlimidazol-2-yl)phenlamine (d(0)-MPIA) and d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl)phenlamine (d(3)-MPIA) have been designed and synthesized. It was successfully used to label aliphatic aldehydes and the aldehyde derivatives were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The new isotope-coded reagents could easily label aldehydes under acidic conditions in the presence of NaCNBH3. The target derivatives exhibited intense [M+H](+) and regular product ions with electrospray ionization source in positive mode. The d(0)/d(3)-MPIA-aldehydes were monitored by the transitions of [M+H](+) -> m/z 322 and [M+H](+) -> m/z 165, and the obtained detection limits were in the range of 0.18-15.9 pg/mL at signal to noise ratio of 3. The global isotope internal standard technology was employed for quantification analysis with d(3)-MPIA-aldehyde as internal standard for corresponding d(0)-MPIA-aldehyde. Excellent linear responses for relative quantification were observed in the range of 1/10-10/1 with coefficients > 0.998. The developed method has been applied to the quantification of aliphatic aldehydes in selected aquatic products with RSD <3.6% and recoveries > 85.2%. (C) 2013 Elsevier B.V. All rights reserved.; A new pair of derivatization reagents, d(0)-4-(1-methyl-1H-phenanthro[9,10-dlimidazol-2-yl)phenlamine (d(0)-MPIA) and d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl)phenlamine (d(3)-MPIA) have been designed and synthesized. It was successfully used to label aliphatic aldehydes and the aldehyde derivatives were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The new isotope-coded reagents could easily label aldehydes under acidic conditions in the presence of NaCNBH3. The target derivatives exhibited intense [M+H](+) and regular product ions with electrospray ionization source in positive mode. The d(0)/d(3)-MPIA-aldehydes were monitored by the transitions of [M+H](+) -> m/z 322 and [M+H](+) -> m/z 165, and the obtained detection limits were in the range of 0.18-15.9 pg/mL at signal to noise ratio of 3. The global isotope internal standard technology was employed for quantification analysis with d(3)-MPIA-aldehyde as internal standard for corresponding d(0)-MPIA-aldehyde. Excellent linear responses for relative quantification were observed in the range of 1/10-10/1 with coefficients > 0.998. The developed method has been applied to the quantification of aliphatic aldehydes in selected aquatic products with RSD <3.6% and recoveries > 85.2%. (C) 2013 Elsevier B.V. All rights reserved. |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/4250]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Sun, Zhiwei,Wang, Xiaoxiang,Cai, Yiping,et al. Development of a pair of differential H/D isotope-coded derivatization reagents d(0)/d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl) phenlamine and its application for determination of aldehydes in selected aquatic products by liquid chromatography-tandem mass spectrometry.[J]. TALANTA, Sun, ZW; Wang, XX; Cai, YP; Fu, JQ; You, JM.Development of a pair of differential H/D isotope-coded derivatization reagents d(0)/d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl) phenlamine and its application for determination of aldehydes in selected aquatic products by liquid chromatography-tandem mass spectrometry.,TALANTA,2014,120():84,2014. |
| APA | Sun, Zhiwei,Wang, Xiaoxiang,Cai, Yiping,Fu, Junqing,&You, Jinmao.(2014).Development of a pair of differential H/D isotope-coded derivatization reagents d(0)/d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl) phenlamine and its application for determination of aldehydes in selected aquatic products by liquid chromatography-tandem mass spectrometry..TALANTA. |
| MLA | Sun, Zhiwei,et al."Development of a pair of differential H/D isotope-coded derivatization reagents d(0)/d(3)-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl) phenlamine and its application for determination of aldehydes in selected aquatic products by liquid chromatography-tandem mass spectrometry.".TALANTA (2014). |
入库方式： OAI收割
来源：西北高原生物研究所
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