Identification and quantification of flavonoid aglycones in rape bee pollen from Qinghai-Tibetan Plateau by HPLC-DAD-APCI/MS
文献类型：期刊论文
| 作者 | Lv, Huanhuan ; Wang, Xiaoyan ; He, Yanfeng ; Wang, Honglun ; Suo, Yourui ; Wang, XY (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China. |
| 刊名 | JOURNAL OF FOOD COMPOSITION AND ANALYSIS
 |
| 出版日期 | 2015-03-01 |
| 英文摘要 | For identification and quantification of flavonoid aglycones in rape bee pollen (RBP) collected from the Qinghai-Tibetan Plateau, a high-performance liquid chromatography (HPLC) separation method with diode array detector (DAD) and atmospheric pressure chemical ionization/mass spectrometric (APCI/MS) detection and four extraction methods (i.e. microwave-assisted extraction, Soxhlet extraction, cold-soaked extraction, and heat reflux extraction) were developed in this study. The identification of flavonoid aglycones was based on retention time and mass spectra by comparison with standards. Results demonstrated that this method showed excellent reproducibility and correlation coefficient, and offered the detection limits of 0.77-15.50 pmol at signal-to-noise ratio of 3. Quercetin and kaempferol were presented in RBP, and microwave-assisted extraction (MAE) was superior to the other three methods in terms of efficiency, convenience and high content of quercetin (1.37 +/- 0.059 mg/g) and kaempferol (23.44 +/- 0.544 mg/g). Our work indicated that: 1) the proposed HPLC-DAD-APCl/MS was an accurate and precise analysis method to identify and quantify the flavonoid aglycones in RBP; and 2) MAE was efficient to extract flavonoids from RBP with short extraction time, low solvent consumption, and homogeneous extraction conditions. (C) 2014 Elsevier Inc. All rights reserved.; For identification and quantification of flavonoid aglycones in rape bee pollen (RBP) collected from the Qinghai-Tibetan Plateau, a high-performance liquid chromatography (HPLC) separation method with diode array detector (DAD) and atmospheric pressure chemical ionization/mass spectrometric (APCI/MS) detection and four extraction methods (i.e. microwave-assisted extraction, Soxhlet extraction, cold-soaked extraction, and heat reflux extraction) were developed in this study. The identification of flavonoid aglycones was based on retention time and mass spectra by comparison with standards. Results demonstrated that this method showed excellent reproducibility and correlation coefficient, and offered the detection limits of 0.77-15.50 pmol at signal-to-noise ratio of 3. Quercetin and kaempferol were presented in RBP, and microwave-assisted extraction (MAE) was superior to the other three methods in terms of efficiency, convenience and high content of quercetin (1.37 +/- 0.059 mg/g) and kaempferol (23.44 +/- 0.544 mg/g). Our work indicated that: 1) the proposed HPLC-DAD-APCl/MS was an accurate and precise analysis method to identify and quantify the flavonoid aglycones in RBP; and 2) MAE was efficient to extract flavonoids from RBP with short extraction time, low solvent consumption, and homogeneous extraction conditions. (C) 2014 Elsevier Inc. All rights reserved. |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5453]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Lv, Huanhuan,Wang, Xiaoyan,He, Yanfeng,et al. Identification and quantification of flavonoid aglycones in rape bee pollen from Qinghai-Tibetan Plateau by HPLC-DAD-APCI/MS[J]. JOURNAL OF FOOD COMPOSITION AND ANALYSIS,2015. |
| APA | Lv, Huanhuan,Wang, Xiaoyan,He, Yanfeng,Wang, Honglun,Suo, Yourui,&Wang, XY .(2015).Identification and quantification of flavonoid aglycones in rape bee pollen from Qinghai-Tibetan Plateau by HPLC-DAD-APCI/MS.JOURNAL OF FOOD COMPOSITION AND ANALYSIS. |
| MLA | Lv, Huanhuan,et al."Identification and quantification of flavonoid aglycones in rape bee pollen from Qinghai-Tibetan Plateau by HPLC-DAD-APCI/MS".JOURNAL OF FOOD COMPOSITION AND ANALYSIS (2015). |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。