3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification
文献类型：期刊论文
| 作者 | You, Jinmao ; Dou, Kun ; Song, Cuihua ; Li, Guoliang ; Sun, Zhiwei ; Zhang, Shijuan ; Chen, Guang ; Zhao, Xianen ; Hu, Na ; Zhou, Wu |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2017-06-01 |
| 英文摘要 | A rapid, sensitive, and selective precolumn derivatization method for the simultaneous determination of eight thiophenols using 3-(2-bromoacetamido)-N-(9-ethyl-9H)-carbazol as a labeling reagent by high-performance liquid chromatography with fluorescence detection has been developed. The labeling reagent reacted with thiophenols at 50 degrees C for 50 min in aqueous acetonitrile in the presence of borate buffer (0.10mol/L, pH 11.2) to give high yields of thiophenol derivatives. The derivatives were identified by online postcolumn mass spectrometry. The collision-induced dissociation spectra for thiophenol derivatives gave the corresponding specific fragment ions at m/z 251.3, 223.3, 210.9, 195.8, and 181.9. At the same time, derivatives exhibited intense fluorescence with an excitation maximum at. ex = 276 nm and an emission maximum at lambda(em) = 385 nm. Excellent linear responses were observed for all analytes over the range of 0.033-6.66 mu mol/L with correlation coefficients of more than 0.9997. Detection limits were in the range of 0.94-5.77 mu g/L with relative standard deviations of less than 4.54%. The feasibility of derivatization allowed the development of a rapid and highly sensitive method for the quantitative analysis of trace levels of thiophenols from some rubber products. The average recoveries (n = 3) were in the range of 87.21-101.12%. |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/7083]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | You, Jinmao,Dou, Kun,Song, Cuihua,et al. 3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification[J]. JOURNAL OF SEPARATION SCIENCE,2017. |
| APA | You, Jinmao.,Dou, Kun.,Song, Cuihua.,Li, Guoliang.,Sun, Zhiwei.,...&Zhou, Wu.(2017).3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification.JOURNAL OF SEPARATION SCIENCE. |
| MLA | You, Jinmao,et al."3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification".JOURNAL OF SEPARATION SCIENCE (2017). |
入库方式： OAI收割
来源：西北高原生物研究所
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