A sensitive fluorescence reagent for the determination of aldehydes from alcoholic beverage using high-performance liquid chromatography with fluorescence detection and mass spectrometric identification
文献类型：期刊论文
| 作者 | You, Jinmao1; Yan, Tao2; Zhao, Huaixin2; Sun, Zhiwei1; Xia, Lian1; Suo, Yourui1; Li, Yulin1 |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2009-03-16 |
| 卷号 | 636期号:1页码:95-104 |
| 关键词 | Column liquid chromatography Aldehydes 2-[2-(7H-dibenzo[a,g] carbazol-7-yl)-ethoxyl ethyl carbonylhydrazine (DBCEEC) Mass spectrometry |
| ISSN号 | 0003-2670 |
| 中文摘要 | a pre-column derivatization method for the sensitive determination of aldehydes using the tagging reagent 2-[2-(7h-dibenzo[a,g] carbazol-7-yl)-ethoxyl ethyl carbonylhydrazine (dbceec) followed by high-performance liquid chromatography with fluorescence detection and apci-ms identification has been developed. the chromophore of fluoren-9-methoxy-carbonylhydrazine (fmoc-hydrazine) reagent was replaced by 2-[2-(7h-dibenzo[a,g] carbazol-7-yl)-ethoxyl ethyl functional group, which resulted in a sensitive fluorescence tagging reagent dbceec. dbceec could easily and quickly labeled aldehydes. the maximum excitation (300 nm) and emission (400 nm) wavelengths did not essentially change for all the aldehyde derivatives. derivatives were sufficiently stable to be efficiently analyzed by high-performance liquid chromatography. the derivatives showed an intense protonated molecular ion corresponding m/z [m + (ch(2))(n)](+) in positive-ion mode (m: molecular weight of dbceec, n: corresponding aldehyde carbon atom numbers). the collision-induced dissociation of protonated molecular ion formed fragment ions at m/z 294.6, m/z 338.6 and m/z 356.5. studies on derivatization demonstrated excellent derivative yields in the presence of trichloroacetic acid (tca) catalyst. maximal yields close to 100% were observed with a 10 to 15-fold molar reagent excess. separation of the derivatized aldehydes had been optimized on zorbax eclipse xdb-c(8) column with aqueous acetonitrile as mobile phase in conjunction with a binary gradient elution. excellent linear responses were observed at the concentration range of 0.01-10 nmol ml(-1) with coefficients of >0.9991. detection limits obtained by the analysis of a derivatized standard containing 0.01 nmol ml(-1) of each aldehyde, were from 0.2 to 1.78 nmol l(-1) (at a signal-to-noise ratio of 3). (c) 2009 elsevier b.v. all rights reserved. |
| 英文摘要 | a pre-column derivatization method for the sensitive determination of aldehydes using the tagging reagent 2-[2-(7h-dibenzo[a,g] carbazol-7-yl)-ethoxyl ethyl carbonylhydrazine (dbceec) followed by high-performance liquid chromatography with fluorescence detection and apci-ms identification has been developed. the chromophore of fluoren-9-methoxy-carbonylhydrazine (fmoc-hydrazine) reagent was replaced by 2-[2-(7h-dibenzo[a,g] carbazol-7-yl)-ethoxyl ethyl functional group, which resulted in a sensitive fluorescence tagging reagent dbceec. dbceec could easily and quickly labeled aldehydes. the maximum excitation (300 nm) and emission (400 nm) wavelengths did not essentially change for all the aldehyde derivatives. derivatives were sufficiently stable to be efficiently analyzed by high-performance liquid chromatography. the derivatives showed an intense protonated molecular ion corresponding m/z [m + (ch(2))(n)](+) in positive-ion mode (m: molecular weight of dbceec, n: corresponding aldehyde carbon atom numbers). the collision-induced dissociation of protonated molecular ion formed fragment ions at m/z 294.6, m/z 338.6 and m/z 356.5. studies on derivatization demonstrated excellent derivative yields in the presence of trichloroacetic acid (tca) catalyst. maximal yields close to 100% were observed with a 10 to 15-fold molar reagent excess. separation of the derivatized aldehydes had been optimized on zorbax eclipse xdb-c(8) column with aqueous acetonitrile as mobile phase in conjunction with a binary gradient elution. excellent linear responses were observed at the concentration range of 0.01-10 nmol ml(-1) with coefficients of >0.9991. detection limits obtained by the analysis of a derivatized standard containing 0.01 nmol ml(-1) of each aldehyde, were from 0.2 to 1.78 nmol l(-1) (at a signal-to-noise ratio of 3). (c) 2009 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | derivatization reagent ; carbonyl-compounds ; quantitative-determination ; electrospray-ionization ; aliphatic-aldehydes ; formaldehyde ; samples ; air ; 2,4-dinitrophenylhydrazones ; separation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264212100015 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1892]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Jinmao,Yan, Tao,Zhao, Huaixin,et al. A sensitive fluorescence reagent for the determination of aldehydes from alcoholic beverage using high-performance liquid chromatography with fluorescence detection and mass spectrometric identification[J]. analytica chimica acta,2009,636(1):95-104. |
| APA | You, Jinmao.,Yan, Tao.,Zhao, Huaixin.,Sun, Zhiwei.,Xia, Lian.,...&Li, Yulin.(2009).A sensitive fluorescence reagent for the determination of aldehydes from alcoholic beverage using high-performance liquid chromatography with fluorescence detection and mass spectrometric identification.analytica chimica acta,636(1),95-104. |
| MLA | You, Jinmao,et al."A sensitive fluorescence reagent for the determination of aldehydes from alcoholic beverage using high-performance liquid chromatography with fluorescence detection and mass spectrometric identification".analytica chimica acta 636.1(2009):95-104. |
入库方式： OAI收割
来源：西北高原生物研究所
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