Enhancing Sensitivity for Atmospheric Pressure Chemical Ionization Mass Spectrometry with a Novel Labeling Reagent: Application for the Analysis of Estrogens Derivatives
文献类型：期刊论文
| 作者 | Zhao Huai-Xin1; Sun Xue-Jun1; Sun Zhi-Wei1; Hu Bao-Jun1; Liu Qin-Ze1; Zhao Xin-Quan2; You Jin-Mao1,2 |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2009-02-01 |
| 卷号 | 37期号:2页码:187-193 |
| 关键词 | Liquid chromatography-mass spectrography 10-ethyl-acridine-2-sulfonyl chloride estradiol estriol |
| ISSN号 | 0253-3820 |
| 中文摘要 | a novel labeling reagent 10-ethyl-acridine-2-sulfonyl chloride (easc) was synthesized for the analysis of trace phenolic compound and a new highly sensitive method for the determination of estrogens such as estradiol and estriol using easc as enhancing reagent with lc-apci ms/ms (mrm) detection has been developed. estradiol (extradiol) and estriol (extriol) could be labeled within 3 min at 60 degrees c in the presence of ph = 10. 5 nahco(3) buffer to give the corresponding sensitive ms ion current signal that could be detected by ms-apci(mrm) in positive-ion mode. compared with dansyl chloride, easc exhibited excellent uv, fluourscence and ms properties: ratio of molar absorptivity uv(easc)/uv(dansyl-cl) = 6. 67, ratio of fluorescence quantum yield phi(f)(easc)/phi(f) (dansyl-cl) = 43, ratio of ion intensity ic(easc)/ic(dansyl-cl) = 4. 98 (extradiol); ic(easc)/ic(dansyl-cl) = 4. 51 (extriol) (at the same derivative condition with extradiol and extriol at the same time, a detailed statement of the enhancing mechanism using easc as ms labeling reagent was explained. the established method exhibited excellent repeatability and recovery. linear responses in the range of 2.0 mu g/l - 100 mu g/l, for extradiol and extriol were observed with coefficients of >0.9995, and detection limits were 4. 3 and 14 ng/l (s/n = 3 : 1) for the labeled extradiol and extriol. the established method for the determination of trace free estradiol and estriol from feces sample of tibetan antelope was satisfactory. |
| 英文摘要 | a novel labeling reagent 10-ethyl-acridine-2-sulfonyl chloride (easc) was synthesized for the analysis of trace phenolic compound and a new highly sensitive method for the determination of estrogens such as estradiol and estriol using easc as enhancing reagent with lc-apci ms/ms (mrm) detection has been developed. estradiol (extradiol) and estriol (extriol) could be labeled within 3 min at 60 degrees c in the presence of ph = 10. 5 nahco(3) buffer to give the corresponding sensitive ms ion current signal that could be detected by ms-apci(mrm) in positive-ion mode. compared with dansyl chloride, easc exhibited excellent uv, fluourscence and ms properties: ratio of molar absorptivity uv(easc)/uv(dansyl-cl) = 6. 67, ratio of fluorescence quantum yield phi(f)(easc)/phi(f) (dansyl-cl) = 43, ratio of ion intensity ic(easc)/ic(dansyl-cl) = 4. 98 (extradiol); ic(easc)/ic(dansyl-cl) = 4. 51 (extriol) (at the same derivative condition with extradiol and extriol at the same time, a detailed statement of the enhancing mechanism using easc as ms labeling reagent was explained. the established method exhibited excellent repeatability and recovery. linear responses in the range of 2.0 mu g/l - 100 mu g/l, for extradiol and extriol were observed with coefficients of >0.9995, and detection limits were 4. 3 and 14 ng/l (s/n = 3 : 1) for the labeled extradiol and extriol. the established method for the determination of trace free estradiol and estriol from feces sample of tibetan antelope was satisfactory. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; dansyl chloride ; electrospray-ionization ; derivatization ; estradiol ; plasma ; serum |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265169000005 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1896]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xinning 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao Huai-Xin,Sun Xue-Jun,Sun Zhi-Wei,et al. Enhancing Sensitivity for Atmospheric Pressure Chemical Ionization Mass Spectrometry with a Novel Labeling Reagent: Application for the Analysis of Estrogens Derivatives[J]. chinese journal of analytical chemistry,2009,37(2):187-193. |
| APA | Zhao Huai-Xin.,Sun Xue-Jun.,Sun Zhi-Wei.,Hu Bao-Jun.,Liu Qin-Ze.,...&You Jin-Mao.(2009).Enhancing Sensitivity for Atmospheric Pressure Chemical Ionization Mass Spectrometry with a Novel Labeling Reagent: Application for the Analysis of Estrogens Derivatives.chinese journal of analytical chemistry,37(2),187-193. |
| MLA | Zhao Huai-Xin,et al."Enhancing Sensitivity for Atmospheric Pressure Chemical Ionization Mass Spectrometry with a Novel Labeling Reagent: Application for the Analysis of Estrogens Derivatives".chinese journal of analytical chemistry 37.2(2009):187-193. |
入库方式： OAI收割
来源：西北高原生物研究所
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