Transcriptomic profiling of Tibetan highland fish (Gymnocypris przewalskii) in response to the infection of parasite ciliate Ichthyophthirius multifiliis
文献类型：期刊论文
| 作者 | Zhao, Kai2,3,4 ; Wanghe, Kunyuan1,2,3,4; Feng, Chenguang1,2,3,4; Tong, Chao1,2,3,4; Tian, Fei2,3,4
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2017-11-01 |
| 卷号 | 70页码:524-535 |
| 关键词 | Gymnocypris Przewalskii Ichthyophthirius Multifiliis Parasite Infection Rna-seq Immune Responses |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2017.09.003 |
| 文献子类 | Article |
| 英文摘要 | Gymnocypris przewalskii is a native cyprinid in the Lake Qinghai of the Qinghai-Tibetan Plateau. G. przewalskii is highly susceptible to the infection of a parasite, Ichthyophthirius multifiliis, in the artificial propagation and breeding. To better understand the host immune reaction to I. multifiliis infection, we characterize the gene expression profiles in the spleen of healthy and I. multifiliis infected G. przewalskii by RNA-seq. Totally, the transcriptomic analysis produces 463,031,110 high quality reads, which are assembled to 213,538 genes with N50 of 1918 bp and the average length of 1205 bp. Of assembled genes, 90.52% are annotated by public databases. The expression analysis shows 744 genes are significantly changed by the infection of I. multifiliis, which are validated by qRT-PCR with the correlation coefficient of 0.896. The differentially expressed genes are classified into 689 GO terms and 230 KEGG pathways, highlighting the promoted innate immunity in I. multifiliis infected G. przewalskii at 2 days post infection. Our results pinpoint that the up-regulated genes are enriched in TLR signaling pathway, inflammatory response and activation of immune cell migration. On the contrary, complement genes are down regulated, indicating the evasion of host complement cascades by I. multifiliis. The repressed genes are also enriched in the pathways related to metabolism and endocrine, suggesting the metabolic disturbance in I. multifiliis treated G. przewalskii. In summary, the present study profiles the gene expression signature of G. przewalskii in the responses to I. multifiliis infection, and improves our understanding on molecular mechanisms of host-parasite interaction in G. przewalskii, which focuses the crucial function of TLRs, cytokines and complement components in the host defense against I. multifiliis. (C) 2017 Elsevier Ltd. All rights reserved. |
| WOS关键词 | ZEBRAFISH DANIO-RERIO ; CARP CYPRINUS-CARPIO ; TOLL-LIKE RECEPTORS ; ONCORHYNCHUS-MYKISS WALBAUM ; IMMUNE-RELEVANT GENES ; INNATE IMMUNITY ; RAINBOW-TROUT ; CHANNEL CATFISH ; EXPRESSION ANALYSIS ; COMPLEMENT EVASION |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000416186800055 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 资助机构 | National Natural Science Foundation of China(31700325) ; Science Foundation of Qinghai Province(2016-ZJ-941Q) ; Construction Fund for Qinghai Key Laboratories(2017-41-Y23) ; "1000 Talent" programs of Qinghai Province |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/13272]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 通讯作者 | Zhao, Kai |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Qinghai Key Lab Anim Ecol Genom, Xining, Qinghai, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Lab Plateau Fish Evolutionary & Funct Genom, Xining, Qinghai, Peoples R China 4.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Qinghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Kai,Wanghe, Kunyuan,Feng, Chenguang,et al. Transcriptomic profiling of Tibetan highland fish (Gymnocypris przewalskii) in response to the infection of parasite ciliate Ichthyophthirius multifiliis[J]. FISH & SHELLFISH IMMUNOLOGY,2017,70:524-535. |
| APA | Zhao, Kai,Wanghe, Kunyuan,Feng, Chenguang,Tong, Chao,&Tian, Fei.(2017).Transcriptomic profiling of Tibetan highland fish (Gymnocypris przewalskii) in response to the infection of parasite ciliate Ichthyophthirius multifiliis.FISH & SHELLFISH IMMUNOLOGY,70,524-535. |
| MLA | Zhao, Kai,et al."Transcriptomic profiling of Tibetan highland fish (Gymnocypris przewalskii) in response to the infection of parasite ciliate Ichthyophthirius multifiliis".FISH & SHELLFISH IMMUNOLOGY 70(2017):524-535. |
入库方式： OAI收割
来源：西北高原生物研究所
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