A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging
文献类型：期刊论文
| 作者 | You, Jinmao1,2 ; Kong, Rongmei2; Qu, Fengli2; Dou, Yanan2; Qu, Fei2; Xia, Lian2; Yang, Daoshan2; Guo, Lan2
|
| 刊名 | NEW JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2017-12-21 |
| 卷号 | 41期号:24页码:15277-15282 |
| ISSN号 | 1144-0546 |
| DOI | 10.1039/c7nj02391j |
| 文献子类 | Article |
| 英文摘要 | In this study, a coumarin-based probe (probe 1) bearing a maleimide group was used to rapidly and selectively detect thiols. After inducing thiols, this probe presented a significant fluorescence enhancement (>22-fold), whereas other amino acids that had no sulfhydryl group just generated slight changes. The detection limits were determined to be as low as 7.41 nM for Cys, 6.06 nM for GSH, and 7.73 nM for Hcy based on S/N = 3. Furthermore, this probe possessed excellent water solubility and successfully detected thiols in PBS (pH 7.4, 99.5%). More importantly, the practical application of probe 1 for the selective detection of thiols was successfully demonstrated in living T24 cells. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; LIVING CELLS ; CAPILLARY-ELECTROPHORESIS ; SELECTIVE DETECTION ; METHYLMALONIC ACID ; HUMAN PLASMA ; IN-VIVO ; CYSTEINE ; HOMOCYSTEINE ; GLUTATHIONE |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000417058800063 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | Natural Science Foundation of Shandong Province(ZR2014BM029) ; National Natural Science Foundation of China(21505084 ; 21375076 ; 21475075 ; 21405093) |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/13284]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 通讯作者 | Xia, Lian |
| 作者单位 | 1.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining, Qinghai, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Jinmao,Kong, Rongmei,Qu, Fengli,et al. A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging[J]. NEW JOURNAL OF CHEMISTRY,2017,41(24):15277-15282. |
| APA | You, Jinmao.,Kong, Rongmei.,Qu, Fengli.,Dou, Yanan.,Qu, Fei.,...&Guo, Lan.(2017).A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging.NEW JOURNAL OF CHEMISTRY,41(24),15277-15282. |
| MLA | You, Jinmao,et al."A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging".NEW JOURNAL OF CHEMISTRY 41.24(2017):15277-15282. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。