中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae)

文献类型:期刊论文

作者Li, Zhong-Hu1,2; Wen, Hong-Yan3; Chen, Jian1; Wu, Gui-Li1; Wang, Yu-Jin1,2
刊名conservation genetics
出版日期2009-06-01
卷号10期号:3页码:747-749
关键词Codonopsis pilosula Nannf. Microsatellite markers Genetic diversity
ISSN号1566-0621
中文摘要codonopsis pilosula nannf., as an important traditional chinese medicinal plant species, has been used to treat fatigue, thirst and loss of appetite. in this study, we developed 10 new microsatellite loci primers from the genome of this species using the combined biotin capture method. the polymorphism of each locus was further assessed through 27 individuals from four geographically distant populations. the number of alleles per locus ranged from 6 to 11. the observed and expected heterozygosity ranged from 0.15 to 0.24 and 0.27 to 0.40, respectively. we further performed cross-priming tests of these loci in the other three congeneric species. these microsatellite markers will be useful for investigating genetic diversity within and between populations of these species.
英文摘要codonopsis pilosula nannf., as an important traditional chinese medicinal plant species, has been used to treat fatigue, thirst and loss of appetite. in this study, we developed 10 new microsatellite loci primers from the genome of this species using the combined biotin capture method. the polymorphism of each locus was further assessed through 27 individuals from four geographically distant populations. the number of alleles per locus ranged from 6 to 11. the observed and expected heterozygosity ranged from 0.15 to 0.24 and 0.27 to 0.40, respectively. we further performed cross-priming tests of these loci in the other three congeneric species. these microsatellite markers will be useful for investigating genetic diversity within and between populations of these species.
WOS标题词science & technology ; life sciences & biomedicine
类目[WOS]biodiversity conservation ; genetics & heredity
研究领域[WOS]biodiversity & conservation ; genetics & heredity
收录类别SCI
语种英语
WOS记录号WOS:000265832000060
公开日期2011-12-13
源URL[http://ir.nwipb.ac.cn//handle/363003/1868]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
作者单位1.Lanzhou Univ, Key Lab Arid & Grassland Ecol, Sch Life Sci, Lanzhou 730000, Peoples R China
2.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Plateau Inst Biol, Xining 810008, Peoples R China
3.Management Off Innovat & Dev Biol Resources, Lijiang 674100, Yunnan, Peoples R China
推荐引用方式
GB/T 7714
Li, Zhong-Hu,Wen, Hong-Yan,Chen, Jian,et al. Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae)[J]. conservation genetics,2009,10(3):747-749.
APA Li, Zhong-Hu,Wen, Hong-Yan,Chen, Jian,Wu, Gui-Li,&Wang, Yu-Jin.(2009).Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae).conservation genetics,10(3),747-749.
MLA Li, Zhong-Hu,et al."Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae)".conservation genetics 10.3(2009):747-749.

入库方式: OAI收割

来源:西北高原生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。