Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae)
文献类型：期刊论文
| 作者 | Li, Ke-xin1,2; Geng, Jia-ning2,3; Yang, Jin3; Zhang, Yan-ming1; Hu, Song-nian3 |
| 刊名 | conservation genetics
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 10期号:3页码:785-787 |
| 关键词 | Magnetic beads enrichment Microsatellite Parentage analysis Plateau pika Polymorphism |
| ISSN号 | 1566-0621 |
| 中文摘要 | high quality jumping microsatellite libraries of the plateau pika (ochotona curzoniae) were constructed; 231 out of the 288 clones contained microsatellite repeat motif and 120 pairs of primers were designed accordingly. polymorphism was assessed for 48 individuals. only 13 microsatellite loci were polymorphic with high polymorphism information content (pic) ranging from 0.598 to 0.871, a condition probably resulting from individuals used for assessment being very closely related. this degree of polymorphism, however, is sufficient to conduct parentage analysis. expected and observed heterozygosities ranged from 0.219 to 0.935 and 0.659 to 0.890, respectively. one locus was in linkage disequilibrium. this information provides efficient tools to allow future parentage studies. |
| 英文摘要 | high quality jumping microsatellite libraries of the plateau pika (ochotona curzoniae) were constructed; 231 out of the 288 clones contained microsatellite repeat motif and 120 pairs of primers were designed accordingly. polymorphism was assessed for 48 individuals. only 13 microsatellite loci were polymorphic with high polymorphism information content (pic) ranging from 0.598 to 0.871, a condition probably resulting from individuals used for assessment being very closely related. this degree of polymorphism, however, is sufficient to conduct parentage analysis. expected and observed heterozygosities ranged from 0.219 to 0.935 and 0.659 to 0.890, respectively. one locus was in linkage disequilibrium. this information provides efficient tools to allow future parentage studies. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biodiversity conservation ; genetics & heredity |
| 研究领域[WOS] | biodiversity & conservation ; genetics & heredity |
| 关键词[WOS] | software |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265832000069 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1872]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Plateau Inst Biol, Xining 810001, Qinghai, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Chinese Acad Sci, Key Lab Genome Informat & Sci, Beijing Inst Genom, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Ke-xin,Geng, Jia-ning,Yang, Jin,et al. Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae)[J]. conservation genetics,2009,10(3):785-787. |
| APA | Li, Ke-xin,Geng, Jia-ning,Yang, Jin,Zhang, Yan-ming,&Hu, Song-nian.(2009).Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae).conservation genetics,10(3),785-787. |
| MLA | Li, Ke-xin,et al."Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae)".conservation genetics 10.3(2009):785-787. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。