Determination of Free Aliphatic Amines in Environmental Water by EASC Derivatization and Identification with LC-APCI-MS
文献类型：期刊论文
| 作者 | Zhao Huai-Xin1; Sun Xue-Jun1; Sun Zhi-Wei2,3; Yan Tao1; Zhang Zai-Qing1; Liu Qin-Ze1; Suo You-Rui2; Li Yu-Lin2; You Jin-Mao1,2 |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2009-04-10 |
| 卷号 | 30期号:4页码:675-681 |
| 关键词 | High performance liquid chromatography/ion-trap mass spectrometry Fluorescence detection Pre-column derivatization 10-ethyl-9-oxo-9, 10-dihydroacridine-2-sulfonyl chloride Aliphatic amine |
| ISSN号 | 0251-0790 |
| 中文摘要 | on a reversed-phase hypersil bds c(18) (4.6 mm x 200 mm, 10 mu m i.d.) column, 12 aliphatic amines were derivatized using 10-ethyl-9-oxo-9,10-dihydroacriditie-2-sulfonyl chloride(easc) as a new pre-column derivatization reagent. the complete baseline resolution was obtained in conjunction with a gradient elution with acetonitrile/h(2)o as mobile phase within 20 min. the optimum excitation and emission wavelengths were at lambda(ex)/lambda(em) = 270 nm/430 nm. comparing with the widely used dansyl-cl, easc has stronger photoluminescence characteristics(ultraviolet and fluorescence) : uv(easc)/uv(dansyl-cl) = 3.2 (uv: ultraviolet absorption intensity); i(easc/i(dansyl-cl) = 30.0: 1-105. 4:1 (i: relative fluorescence intensity); fluorescence quantum yield: phi(f)(easc)/phi(f)(dansyl-cl) = 43.0:1. fast and accurate quantitative analysis of aliphatic amines with fluorescence and corresponding post-column ion trap mass spectrometry(lc-apci-ms) were obtained. easc-amines exhibited stronger mass spectrometric sensitivity relative to that of dansyl-amine derivatives: ms(easc)/ms(dansyl-cl) = 1. 6: 1-6. 2: 1 times(ms: relative intensity of ion current). the established method for the determination of aliphatic amines in environmental water samples exhibited good reproducibility and recovery. excellent linear responses were observed with the correlation coefficients >0.9995. the detection limits(at signal-to-noise of 3: 1) were at levels of 4.0-12.7 fmol. |
| 英文摘要 | on a reversed-phase hypersil bds c(18) (4.6 mm x 200 mm, 10 mu m i.d.) column, 12 aliphatic amines were derivatized using 10-ethyl-9-oxo-9,10-dihydroacriditie-2-sulfonyl chloride(easc) as a new pre-column derivatization reagent. the complete baseline resolution was obtained in conjunction with a gradient elution with acetonitrile/h(2)o as mobile phase within 20 min. the optimum excitation and emission wavelengths were at lambda(ex)/lambda(em) = 270 nm/430 nm. comparing with the widely used dansyl-cl, easc has stronger photoluminescence characteristics(ultraviolet and fluorescence) : uv(easc)/uv(dansyl-cl) = 3.2 (uv: ultraviolet absorption intensity); i(easc/i(dansyl-cl) = 30.0: 1-105. 4:1 (i: relative fluorescence intensity); fluorescence quantum yield: phi(f)(easc)/phi(f)(dansyl-cl) = 43.0:1. fast and accurate quantitative analysis of aliphatic amines with fluorescence and corresponding post-column ion trap mass spectrometry(lc-apci-ms) were obtained. easc-amines exhibited stronger mass spectrometric sensitivity relative to that of dansyl-amine derivatives: ms(easc)/ms(dansyl-cl) = 1. 6: 1-6. 2: 1 times(ms: relative intensity of ion current). the established method for the determination of aliphatic amines in environmental water samples exhibited good reproducibility and recovery. excellent linear responses were observed with the correlation coefficients >0.9995. the detection limits(at signal-to-noise of 3: 1) were at levels of 4.0-12.7 fmol. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; dansyl chloride ; precolumn derivatization ; fluorescence detection ; derivatives ; ionization |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265665700007 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1886]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal Shandony, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao Huai-Xin,Sun Xue-Jun,Sun Zhi-Wei,et al. Determination of Free Aliphatic Amines in Environmental Water by EASC Derivatization and Identification with LC-APCI-MS[J]. chemical journal of chinese universities-chinese,2009,30(4):675-681. |
| APA | Zhao Huai-Xin.,Sun Xue-Jun.,Sun Zhi-Wei.,Yan Tao.,Zhang Zai-Qing.,...&You Jin-Mao.(2009).Determination of Free Aliphatic Amines in Environmental Water by EASC Derivatization and Identification with LC-APCI-MS.chemical journal of chinese universities-chinese,30(4),675-681. |
| MLA | Zhao Huai-Xin,et al."Determination of Free Aliphatic Amines in Environmental Water by EASC Derivatization and Identification with LC-APCI-MS".chemical journal of chinese universities-chinese 30.4(2009):675-681. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。