Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry
文献类型：期刊论文
| 作者 | Jing, Nianhua ; Shi, Junyou ; Li, Guoliang ; Sun, Zhiwei ; You, Jinmao |
| 刊名 | FOOD RESEARCH INTERNATIONAL
 ; Dong, LH; Shi, JY; Liu, YJ.Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry,FOOD RESEARCH INTERNATIONAL,2012,48(1):155-163
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| 出版日期 | 2012-08-01 |
| 英文摘要 | Owing to the growing consumer interest in low-fat foods, it is necessary to provide our customers with the information about the fatty acid composition of mushrooms. In this study, a selective and sensitive method based on pre-column derivatization using 2-(12-oxobenzo[b]-acridin-5(12H)-yl)-ethyl-4-toluenesulfonate (BAETS) as the labeling reagent has been optimized by high-performance liquid chromatography with fluorescence detection and online mass spectrometry identification (HPLC-FLD-MS/MS). Fatty acids (FA) were derivatized by BAETS and separated on a reversed-phase Hypersil BDS C8 column with a gradient elution. Eighteen FA investigated were found to give excellent linear responses with correlation coefficients of >0.9996. Limits of detection and quantification (LOD and LOQ) were in the range of 0.46 to 1.02 ng mL(-1) and 1.43 to 3.48 ng mL(-1), respectively. This method was applied to the quantitative analysis of FA from a wild and four cultivated mushroom species. The wild mushroom named Armillaria luteo-virens contained higher unsaturated fatty acids (UFA) when compared to the cultivated species (Flammulina velutiper, Pleurotus eryngii, Copyinds comatus and Agrocybe aegerita). Ratio of UFA:SFA (SFA, saturated fatty acids) for A. luteo-virens was >5 whereas the values for the cultivated species were <4.09. Therefore, BAETS derivatization allowed the development of a highly sensitive and specific method for the determination of FA in mushroom samples. (C) 2012 Elsevier Ltd. All rights reserved.; Owing to the growing consumer interest in low-fat foods, it is necessary to provide our customers with the information about the fatty acid composition of mushrooms. In this study, a selective and sensitive method based on pre-column derivatization using 2-(12-oxobenzo[b]-acridin-5(12H)-yl)-ethyl-4-toluenesulfonate (BAETS) as the labeling reagent has been optimized by high-performance liquid chromatography with fluorescence detection and online mass spectrometry identification (HPLC-FLD-MS/MS). Fatty acids (FA) were derivatized by BAETS and separated on a reversed-phase Hypersil BDS C8 column with a gradient elution. Eighteen FA investigated were found to give excellent linear responses with correlation coefficients of >0.9996. Limits of detection and quantification (LOD and LOQ) were in the range of 0.46 to 1.02 ng mL(-1) and 1.43 to 3.48 ng mL(-1), respectively. This method was applied to the quantitative analysis of FA from a wild and four cultivated mushroom species. The wild mushroom named Armillaria luteo-virens contained higher unsaturated fatty acids (UFA) when compared to the cultivated species (Flammulina velutiper, Pleurotus eryngii, Copyinds comatus and Agrocybe aegerita). Ratio of UFA:SFA (SFA, saturated fatty acids) for A. luteo-virens was >5 whereas the values for the cultivated species were <4.09. Therefore, BAETS derivatization allowed the development of a highly sensitive and specific method for the determination of FA in mushroom samples. (C) 2012 Elsevier Ltd. All rights reserved. |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3632]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Jing, Nianhua,Shi, Junyou,Li, Guoliang,et al. Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry[J]. FOOD RESEARCH INTERNATIONAL, Dong, LH; Shi, JY; Liu, YJ.Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry,FOOD RESEARCH INTERNATIONAL,2012,48(1):155-163,2012. |
| APA | Jing, Nianhua,Shi, Junyou,Li, Guoliang,Sun, Zhiwei,&You, Jinmao.(2012).Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry.FOOD RESEARCH INTERNATIONAL. |
| MLA | Jing, Nianhua,et al."Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry".FOOD RESEARCH INTERNATIONAL (2012). |
入库方式： OAI收割
来源：西北高原生物研究所
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