SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC
文献类型：期刊论文
| 作者 | Zhao, Xiao-Hui ; Han, Fa ; Li, Yu-Lin ; Zhou, Guo-Ying ; Yue, Hui-Lan ; Yue, HL (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China. |
| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 ; Zhao, XH; Han, F; Li, YL; Zhou, GY; Yue, HL.SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC,JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2013,36(13):1751-1761
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| 出版日期 | 2013-05-01 |
| 英文摘要 | An effective highspeed countercurrent chromatography (HSCCC) method was established for semi-preparative isolation and purification of bioactive chemical constituents from the Tibetan medicinal plant Pedicularis longiflora var. tubiformis (Klotzsch) P. C. Tsoong. With a two-phase solvent system composed of chloroformmethanolwater (8:4:5, v/v), 40mg of extract was separated to yield luteolin (12.5mg), apigenin (9.6mg), and chrysoeriol (4.8mg), with purities of 99.3, 98.2, and 98.6%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of these three components were identified by H-1 NMR and C-13 NMR.; An effective highspeed countercurrent chromatography (HSCCC) method was established for semi-preparative isolation and purification of bioactive chemical constituents from the Tibetan medicinal plant Pedicularis longiflora var. tubiformis (Klotzsch) P. C. Tsoong. With a two-phase solvent system composed of chloroformmethanolwater (8:4:5, v/v), 40mg of extract was separated to yield luteolin (12.5mg), apigenin (9.6mg), and chrysoeriol (4.8mg), with purities of 99.3, 98.2, and 98.6%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of these three components were identified by H-1 NMR and C-13 NMR. |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3946]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, Xiao-Hui,Han, Fa,Li, Yu-Lin,et al. SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, Zhao, XH; Han, F; Li, YL; Zhou, GY; Yue, HL.SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC,JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2013,36(13):1751-1761,2013. |
| APA | Zhao, Xiao-Hui,Han, Fa,Li, Yu-Lin,Zhou, Guo-Ying,Yue, Hui-Lan,&Yue, HL .(2013).SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES. |
| MLA | Zhao, Xiao-Hui,et al."SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES (2013). |
入库方式： OAI收割
来源：西北高原生物研究所
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