Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim
文献类型：期刊论文
| 作者 | Han, Lijuan ; Suo, Yourui ; Yang, Yongjing ; Meng, Jing ; Hu, Na |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| 出版日期 | 2016-04-01 |
| 英文摘要 | In this study, the extraction of water-soluble polysaccharides (BDPs) from Berberis dasystachya Maxim using dynamic microwave-assisted extraction (DMAE) was discussed. A Box-Behnken design combined with response surface methodology has been employed to optimize extraction parameters of DMAE. The BDPs have been analyzed in order to identify a variety of chemical properties. Antioxidant and anti-tumor activities in vitro have been studied by DPPH, ABTS, reducing power assay, and MTT assay, respectively. The results obtained showed that the optimal extraction conditions were as follows: ratio of water to raw material (X-1) 25.84 mg/L, extraction power (X-2) 433.13 W, extraction time (X-3) 35.18 min, and the maximum yield of extraction was 6.472 +/- 0.384%, which was in good agreement with the predicted value. The physicochemical tests demonstrated that the BDPs mainly consist of rhamnose, arabinose, xylose, mannose, glucose and lactose in a molar ratio of 1:17.3:1.33:7:2.33:1.78; the average molecular weight of the BDPs was estimated to be from 2.95 x 10(5) and 1.52 x 10(3) Da, respectively. Furthermore, the BDPs exhibited effective antioxidant and anti-proliferative properties in vitro. Such pharmaceutical activities could prove useful for potential future applications involving the berries of B. dasystachya Maxim. (C) 2015 Elsevier B.V. All rights reserved. |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5785]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Han, Lijuan,Suo, Yourui,Yang, Yongjing,et al. Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016. |
| APA | Han, Lijuan,Suo, Yourui,Yang, Yongjing,Meng, Jing,&Hu, Na.(2016).Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. |
| MLA | Han, Lijuan,et al."Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2016). |
入库方式： OAI收割
来源：西北高原生物研究所
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