An accurate and highly efficient method for finding antioxidant chemicals from Capsicum annuum L.
文献类型：期刊论文
| 作者 | Zhang, Xiaoqian ; Jiang, Lei ; Ma, Lin ; Mei, Lijuan ; Zhang, Yaozhou ; Mei, LJ (reprint author), Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China. |
| 刊名 | ANALYTICAL METHODS
 |
| 出版日期 | 2015 |
| 英文摘要 | Two-dimensional preparative high-performance liquid chromatography (HPLC) was used on the nonaqueous mobile phase in the separation of weakly polar compounds from Capsicum annuum L. The resulting silica column, coupled with an amide column, showed good orthogonality with the non-aqueous mobile phase, and two separate compounds were efficiently isolated in purity above 97%. The relative antioxidant activities of the purified compounds were assessed by a unique cellular antioxidant activity (CAA) model using an anti-oxidative response element (ARE) to regulate the expression level of the luciferase reporter gene, with tert-butyl hydroquinone (TBHQ) as positive control. A luciferase cellular antioxidant activity unit (LCAA) for the measurement of antioxidant capacity has been defined by the antioxidant capacity of 1.5 mg mL(-1) TBHQ, and the relative antioxidant capacities of two active compounds of interest, Fraction 5-4 and Fraction 5-5, were found to be 0.397 LCAA and 0.523 LCAA, respectively. The protocols described will be of considerable assistance in the development of functional foodstuffs.; Two-dimensional preparative high-performance liquid chromatography (HPLC) was used on the nonaqueous mobile phase in the separation of weakly polar compounds from Capsicum annuum L. The resulting silica column, coupled with an amide column, showed good orthogonality with the non-aqueous mobile phase, and two separate compounds were efficiently isolated in purity above 97%. The relative antioxidant activities of the purified compounds were assessed by a unique cellular antioxidant activity (CAA) model using an anti-oxidative response element (ARE) to regulate the expression level of the luciferase reporter gene, with tert-butyl hydroquinone (TBHQ) as positive control. A luciferase cellular antioxidant activity unit (LCAA) for the measurement of antioxidant capacity has been defined by the antioxidant capacity of 1.5 mg mL(-1) TBHQ, and the relative antioxidant capacities of two active compounds of interest, Fraction 5-4 and Fraction 5-5, were found to be 0.397 LCAA and 0.523 LCAA, respectively. The protocols described will be of considerable assistance in the development of functional foodstuffs. |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5479]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaoqian,Jiang, Lei,Ma, Lin,et al. An accurate and highly efficient method for finding antioxidant chemicals from Capsicum annuum L.[J]. ANALYTICAL METHODS,2015. |
| APA | Zhang, Xiaoqian,Jiang, Lei,Ma, Lin,Mei, Lijuan,Zhang, Yaozhou,&Mei, LJ .(2015).An accurate and highly efficient method for finding antioxidant chemicals from Capsicum annuum L..ANALYTICAL METHODS. |
| MLA | Zhang, Xiaoqian,et al."An accurate and highly efficient method for finding antioxidant chemicals from Capsicum annuum L.".ANALYTICAL METHODS (2015). |
入库方式： OAI收割
来源：西北高原生物研究所
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