The influence of organic sample solvents on the separation efficiency of basic compounds under strong cation exchange mode
文献类型：期刊论文
| 作者 | Long, Zhen ; Yu, Dongping ; Liu, Yanfang ; Du, Nana ; Tao, Yanduo ; Mei, Lijuan ; Guo, Zhimou ; Liang, Xinmiao |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2015-05-04 |
| 英文摘要 | This study investigated the influence of organic sample solvents on separation efficiency of basic compounds under strong cation exchange (SCX) mode. The mixtures of acidic aqueous solution and organic solvent such as acetonitrile, ethanol, methanol and dimethyl sulfoxide (DMSO) were tested as sample solvents. For later-eluting analytes, the increase of sample solvent elution strength was responsible for the decrease of separation efficiency. Thus, sample solvents with weak elution strength could provide high separation efficiencies. For earlier-eluting analytes, the retention of organic sample solvents was the main factor affecting separation efficiency. Weakly retained solvents could provide high separation efficiency. In addition, an optimized approach was proposed to reduce the effect of organic sample solvent, in which low ionic solvent was employed as initial mobile phase in the gradient. At last, the analysis of impurities in hydrophobic drug berberine was performed. The results showed that using acidic aqueous methanol as sample solvents could provide high separation efficiency and good resolution (R > 1.5). (C) 2015 Elsevier B.V. All rights reserved.; This study investigated the influence of organic sample solvents on separation efficiency of basic compounds under strong cation exchange (SCX) mode. The mixtures of acidic aqueous solution and organic solvent such as acetonitrile, ethanol, methanol and dimethyl sulfoxide (DMSO) were tested as sample solvents. For later-eluting analytes, the increase of sample solvent elution strength was responsible for the decrease of separation efficiency. Thus, sample solvents with weak elution strength could provide high separation efficiencies. For earlier-eluting analytes, the retention of organic sample solvents was the main factor affecting separation efficiency. Weakly retained solvents could provide high separation efficiency. In addition, an optimized approach was proposed to reduce the effect of organic sample solvent, in which low ionic solvent was employed as initial mobile phase in the gradient. At last, the analysis of impurities in hydrophobic drug berberine was performed. The results showed that using acidic aqueous methanol as sample solvents could provide high separation efficiency and good resolution (R > 1.5). (C) 2015 Elsevier B.V. All rights reserved. |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5558]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Long, Zhen,Yu, Dongping,Liu, Yanfang,et al. The influence of organic sample solvents on the separation efficiency of basic compounds under strong cation exchange mode[J]. ANALYTICA CHIMICA ACTA,2015. |
| APA | Long, Zhen.,Yu, Dongping.,Liu, Yanfang.,Du, Nana.,Tao, Yanduo.,...&Liang, Xinmiao.(2015).The influence of organic sample solvents on the separation efficiency of basic compounds under strong cation exchange mode.ANALYTICA CHIMICA ACTA. |
| MLA | Long, Zhen,et al."The influence of organic sample solvents on the separation efficiency of basic compounds under strong cation exchange mode".ANALYTICA CHIMICA ACTA (2015). |
入库方式： OAI收割
来源：西北高原生物研究所
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