A new chromatographic approach to analyze methylproteome with enhanced lysine methylation identification performance
文献类型：期刊论文
| 作者 | Wang, Qi1,2; Liu, Zhen1,2; Wang, Keyun1,2; Wang, Yan1,2; Ye, Mingliang1,2 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2019-08-30 |
| 卷号 | 1068页码:111-119 |
| ISSN号 | 0003-2670 |
| 关键词 | Methylproteome Strong cation exchange Immobilized metal ion affinity chromatography High-pH reversed-phase chromatography Lysine methylation identification |
| DOI | 10.1016/j.aca.2019.03.042 |
| 通讯作者 | Ye, Mingliang(mingliang@dicp.ac.cn) |
| 英文摘要 | Arginine/lysine methylation is an important post-translational modification (PTM) involved in DNA repairing, transcriptional regulation, etc. Immunoaffinity enrichment is currently the most widely used methods for the methylproteome analysis. Large-scale analysis of arginine methylation has been realized by using pan-R-methyl antibodies. Unfortunately, pan specific antibodies targeting all three lysine methylation forms are not available. In this study, we presented a novel chromatography-based enrichment method for global methylproteome analysis. The offline multidimensional tandem chromatography combining strong cation exchange (SCX) chromatography, immobilized metal ion affinity chromatography (IMAC) and high-pH reversed-phase chromatography (high-pH RP) was applied in the large-scale analysis of methylproteome. Totally, 860 forms on 765 sites were identified from BEL cells, covering all five arginine/lysine methylation forms. Among them, 27.21% were lysine methylation forms. This technique allows the simultaneous analysis of both arginine and lysine methylation while it has improved performance for the identification of lysine methylation. Therefore, it is a promising strategy for the investigation of biological functions related to methylation. (C) 2019 Elsevier B.V. All rights reserved. |
| WOS关键词 | NONHISTONE PROTEIN METHYLATION ; MASS-SPECTROMETRY ; ARGININE METHYLATION ; GLOBAL ANALYSIS ; ENRICHMENT ; REGULATOR ; PEPTIDES ; STRATEGY |
| 资助项目 | National Key R&D Program of China[2016YFA0501402] ; National Key R&D Program of China[2017YFA0505004] ; National Natural Science Foundation of China[21535008] ; National Natural Science Foundation of China[91753105] ; National Natural Science Foundation of China[21804131] ; National Science Fund of China for Distinguished Young Scholars[21525524] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000466926600013 |
| 资助机构 | National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/165460]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Ye, Mingliang |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Qi,Liu, Zhen,Wang, Keyun,et al. A new chromatographic approach to analyze methylproteome with enhanced lysine methylation identification performance[J]. ANALYTICA CHIMICA ACTA,2019,1068:111-119. |
| APA | Wang, Qi,Liu, Zhen,Wang, Keyun,Wang, Yan,&Ye, Mingliang.(2019).A new chromatographic approach to analyze methylproteome with enhanced lysine methylation identification performance.ANALYTICA CHIMICA ACTA,1068,111-119. |
| MLA | Wang, Qi,et al."A new chromatographic approach to analyze methylproteome with enhanced lysine methylation identification performance".ANALYTICA CHIMICA ACTA 1068(2019):111-119. |
入库方式： OAI收割
来源：大连化学物理研究所
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