Integration of stable isotope labeling derivatization and magnetic dispersive solid phase extraction for measurement of neurosteroids by in vivo microdialysis and UHPLC-MS/MS
文献类型：期刊论文
| 作者 | Zhao, Xian-En2; Xu, Yanqiu2; Sun, Luping2; Wang, Xin2; Zhu, Shuyun2; You, Jinmao2; Bai, Yu1; Liu, Huwei1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2019-07-01 |
| 卷号 | 199页码:97-106 |
| ISSN号 | 0039-9140 |
| 关键词 | Neurosteroids Isotope-coded derivatization Magnetic separation Triple quadrupole mass spectrometry In vivo microdialysis technique Parkinson's disease |
| DOI | 10.1016/j.talanta.2019.02.011 |
| 通讯作者 | Zhao, Xian-En(xianenzhao@163.com) |
| 英文摘要 | In this work, a novel strategy of stable isotope labeling derivatization (SILD) combined with magnetic dispersive solid-phase extraction (MDSPE), has been proposed for simultaneous monitoring of neurosteroids changes linked to Parkinson's disease (PD) by in vivo microdialysis. The developed method was based on ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) detection using multiple-reaction monitoring (MRM) mode. In this study, a new pair of stable isotope labeling reagents d(0)-/d(3)-3-N-methyl-2'-carboxyl Rhodamine 6G (d(0)-/d(3)-MCR6G), were designed and synthesized for derivatizing neurosteroids in rat blood microdialysates and neurosteroid standards, respectively. d(3)-MCR6G labeled neurosteroids standards were used as internal standard for the following pretreatment and UHPLC-MS/MS quantification to minimize the deviations caused by complex matrix and ion suppression effects in mass spectrometry analysis. Under the optimized derivatization and extraction conditions, good linearities of eight neurosteroids were obtained with correlation coefficients R-2 values > 0.98. The limits of detection (LODs) and quantitation (LOQs) ranged from 0.06 to 0.12 pg/mL and 0.30-0.40 pg/mL, respectively. Taken together, the established method exhibited high sensitivity and selectivity, excellent accuracy, convenience and high efficiency. It was applied for the simultaneous and dynamic measurement of multiple neurosteroids in normal and PD rat blood microdialysates. This method would be expected to be potentially useful for the monitoring and drug treatment of PD and related neurological disorders in the future. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; ENVIRONMENTAL WATER SAMPLES ; GREEN SYNTHESIS STRATEGY ; MASS-SPECTROMETRY ; QUANTITATIVE-ANALYSIS ; BIOLOGICAL SAMPLES ; CARBOXYLIC-ACIDS ; GRAPHENE OXIDE ; RAT-BRAIN ; NEUROTRANSMITTERS |
| 资助项目 | National Natural Science Foundation of China[21775088] ; National Natural Science Foundation of China[21527809] ; National Natural Science Foundation of China[21575007] ; National Natural Science Foundation of China[21775008] ; National Natural Science Foundation of China[21405094] ; National Natural Science Foundation of China[81303179] ; Open Research Fund of Beijing National Laboratory for Molecular Sciences[BNLMS20160147] ; Foundation of Qufu Normal University[BSQD2012019] ; Foundation of Qufu Normal University[BSQD2012023] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000465050000014 |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Foundation of Qufu Normal University ; Foundation of Qufu Normal University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Foundation of Qufu Normal University ; Foundation of Qufu Normal University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Foundation of Qufu Normal University ; Foundation of Qufu Normal University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Open Research Fund of Beijing National Laboratory for Molecular Sciences ; Foundation of Qufu Normal University ; Foundation of Qufu Normal University |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/165554]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhao, Xian-En |
| 作者单位 | 1.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xian-En,Xu, Yanqiu,Sun, Luping,et al. Integration of stable isotope labeling derivatization and magnetic dispersive solid phase extraction for measurement of neurosteroids by in vivo microdialysis and UHPLC-MS/MS[J]. TALANTA,2019,199:97-106. |
| APA | Zhao, Xian-En.,Xu, Yanqiu.,Sun, Luping.,Wang, Xin.,Zhu, Shuyun.,...&Liu, Huwei.(2019).Integration of stable isotope labeling derivatization and magnetic dispersive solid phase extraction for measurement of neurosteroids by in vivo microdialysis and UHPLC-MS/MS.TALANTA,199,97-106. |
| MLA | Zhao, Xian-En,et al."Integration of stable isotope labeling derivatization and magnetic dispersive solid phase extraction for measurement of neurosteroids by in vivo microdialysis and UHPLC-MS/MS".TALANTA 199(2019):97-106. |
入库方式： OAI收割
来源：大连化学物理研究所
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