Quantitative determination of bioactive proteins in diphtheria tetanus acellular pertussis (DTaP) vaccine by liquid chromatography tandem mass spectrometry
文献类型：期刊论文
| 作者 | Li, Changkun3; Long, Zhen3; Wei, Chen1; Zhan, Zhaoqi4; Ma, Xiao1; Li, Xiuling2; Li, Yueqi3; Yao, Jinting3; Ji, Feng3; Huang, Taohong3 |
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2019-05-30 |
| 卷号 | 169页码:30-40 |
| ISSN号 | 0731-7085 |
| 关键词 | LC-MS/MS Filamentous hemagglutinin Pertactin Fimbriae Pertussis toxin Diphtheria tetanus acellular pertussis vaccine |
| DOI | 10.1016/j.jpba.2019.02.029 |
| 通讯作者 | Li, Xiuling(lixiuling@dicp.ac.cn) ; Li, Yueqi(fxlyq@shimadzu.com.cn) |
| 英文摘要 | A liquid chromatography tandem mass spectrometry method (LC-MS/MS) was developed to determine simultaneously the bioactive proteins including pertussis toxin (PT) subunits, filamentous hemagglutinin (FHA), pertactin (PRN) and fimbriae (FIM) in diphtheria, tetanus and acellular pertussis combined vaccine (DTaP). The trypsin digestion conditions were investigated in detail using in reference to achieve satisfactory results in detection of the peptides on LC-MS/MS with a Bio-C18 column. The performance of the described method was evaluated using reference proteins and the results showed a wide linear range (0.15-24 ng mu L-1), a high sensitivity (0.038 ng. mu L-1 for FHA) and a good precision (RSD of peak area <3.3%). This novel LC-MS/MS method was applied to determine PT subunits, FHA, PRN and FIM in DTaP vaccines, a total of ten batches, obtained from five manufacturers. The results revealed clearly that batch-to-batch consistency of the DTaP vaccines in terms of the protein amounts was stable, while those from manufacturers were varied significantly. On the other hand, the amount of bioactive proteins in component DTaP vaccines was generally higher than those in co-purified DTaP vaccines. The described LC-MS/MS method was compared with Chinese Pharmacopeia method (Lowry method) and it was found that FHA and PRN amounts measured by the two methods were in good agreement. The LC-MS/MS method could provide the amounts of PT subunits. However, the Lowry method could not differentiate the subunits. The LC-MS/MS method was not only more selective and sensitive, but it can be used to determine simultaneously different bioactive proteins in complex matrix-formulated vaccines. The method was extended successfully in other purposes, such as the effect of detoxification on bioactive proteins and characterization of PT references from four organizations worldwide. (C) 2019 Elsevier B.V. All rights reserved. |
| WOS关键词 | FILAMENTOUS HEMAGGLUTININ ; TOXIN ; QUANTIFICATION ; DETOXIFICATION ; PURIFICATION ; ANTIGENS ; TRENDS |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000464892500004 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/165624]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Li, Xiuling; Li, Yueqi |
| 作者单位 | 1.Minist Hlth Res Qual & Standardizat Biotech Prod, Natl Inst Food & Drug Control, Key Lab, Beijing 102629, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Shimadzu Sci Instrument Co, Beijing 100010, Peoples R China 4.Shimadzu Asia Pacific Pte Ltd, Singapore 118264, Singapore |
| 推荐引用方式 GB/T 7714 | Li, Changkun,Long, Zhen,Wei, Chen,et al. Quantitative determination of bioactive proteins in diphtheria tetanus acellular pertussis (DTaP) vaccine by liquid chromatography tandem mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2019,169:30-40. |
| APA | Li, Changkun.,Long, Zhen.,Wei, Chen.,Zhan, Zhaoqi.,Ma, Xiao.,...&Huang, Taohong.(2019).Quantitative determination of bioactive proteins in diphtheria tetanus acellular pertussis (DTaP) vaccine by liquid chromatography tandem mass spectrometry.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,169,30-40. |
| MLA | Li, Changkun,et al."Quantitative determination of bioactive proteins in diphtheria tetanus acellular pertussis (DTaP) vaccine by liquid chromatography tandem mass spectrometry".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 169(2019):30-40. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。