High Anti-Interfering Profiling of Endogenous Glycopeptides for Article Human Plasma by the Dual-Hydrophilic Metal-Organic Framework
文献类型:期刊论文
作者 | Zhang, Lihua1; Peng, Jiaxi1,2; Hu, Yechen1,2; Zhang, Hongyan1; Wan, Lihong1; Wang, Li1; Liang, Zhen1; Wei, Ren'an1 |
刊名 | ANALYTICAL CHEMISTRY |
出版日期 | 2019-04-02 |
卷号 | 91期号:7页码:4852-4859 |
ISSN号 | 0003-2700 |
DOI | 10.1021/acs.analchem.9b00542 |
通讯作者 | Zhang, Lihua(lihuazhang@dicp.ac.cn) ; Wei, Ren'an(wurenan@dicp.ac.cn) |
英文摘要 | Glycopeptidome profiling provides large-scale information about the glycosylation level of endogenous peptides, reflecting the dynamic processes of disease occurrences and developments. However, endogenous glycopeptides are usually submerged in complex fluids containing a wide variety of interference molecules, such as high concentration proteins, nonglycopeptides, and salts, which confounds attempts to identify glycopeptidome. Here, a dual hydrophilic metal organic framework is developed to selectively capture endogenous glycopeptides in complex biological fluid. The hydrophilic matrix material provides specific selectivity toward glycopeptides, while the deliberate surface regulation using hydrophilic species enhances its interaction with glycopeptides. This hydrophilic probe presents an extremely high performance in anti-interfering enrichment of glycopeptides from mimic complex samples, even when the molar ratio of immunoglobulin G versus bovine serum albumin was up to about 1:5000. More excitingly, in the practical application of glycopeptidome analysis, a total of 380 endogenous N-glycopeptides with 180 unique N-glycopeptide sites were identified from human plasma. This strategy is expected to broaden the application of dual-hydrophilic MOF-based materials, especially in dealing with the challenges of extremely complex biological samples. |
WOS关键词 | SITE-SPECIFIC CHARACTERIZATION ; EFFICIENT ENRICHMENT ; SERUM PEPTIDES ; GLYCOSYLATION ; EXTRACTION ; MECHANISMS ; REVEALS |
资助项目 | National Natural Science Foundation of China[21675156] ; National Natural Science Foundation of China[21375125] ; National Natural Science Foundation of China[21725506] ; National Natural Science Foundation of China[91543201] ; Instrument Developing Project of the Chinese Academy of Sciences[YZ201503] ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; National Key R&D Program of China[2017YFA0505003] ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics[DICP TMSR201601] |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | AMER CHEMICAL SOC |
WOS记录号 | WOS:000463683300078 |
资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/165630] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
通讯作者 | Zhang, Lihua; Wei, Ren'an |
作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Lihua,Peng, Jiaxi,Hu, Yechen,et al. High Anti-Interfering Profiling of Endogenous Glycopeptides for Article Human Plasma by the Dual-Hydrophilic Metal-Organic Framework[J]. ANALYTICAL CHEMISTRY,2019,91(7):4852-4859. |
APA | Zhang, Lihua.,Peng, Jiaxi.,Hu, Yechen.,Zhang, Hongyan.,Wan, Lihong.,...&Wei, Ren'an.(2019).High Anti-Interfering Profiling of Endogenous Glycopeptides for Article Human Plasma by the Dual-Hydrophilic Metal-Organic Framework.ANALYTICAL CHEMISTRY,91(7),4852-4859. |
MLA | Zhang, Lihua,et al."High Anti-Interfering Profiling of Endogenous Glycopeptides for Article Human Plasma by the Dual-Hydrophilic Metal-Organic Framework".ANALYTICAL CHEMISTRY 91.7(2019):4852-4859. |
入库方式: OAI收割
来源:大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。