CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications
文献类型：期刊论文
| 作者 | Cai, Peng1,2; Gao, Jiaoqi1; Zhou, Yongjin1 |
| 刊名 | MICROBIAL CELL FACTORIES
 |
| 出版日期 | 2019-04-02 |
| 卷号 | 18页码:12 |
| 关键词 | CRISPR-Cas9 Non-conventional yeasts Genome editing Guide RNA Homologous recombination Non-homologous end joining |
| ISSN号 | 1475-2859 |
| DOI | 10.1186/s12934-019-1112-2 |
| 通讯作者 | Zhou, Yongjin(zhouyongjin@dicp.ac.cn) |
| 英文摘要 | Non-conventional yeasts are playing important roles as cell factories for bioproduction of biofuels, food additives and proteins with outstanding natural characteristics. However, the precise genome editing is challenging in non-conventional yeasts due to lack of efficient genetic tools. In the past few years, CRISPR-based genome editing worked as a revolutionary tool for genetic engineering and showed great advantages in cellular metabolic engineering. Here, we review the current advances and barriers of CRISPR-Cas9 for genome editing in non-conventional yeasts and propose the possible solutions in enhancing its efficiency for precise genetic engineering. |
| WOS关键词 | METHYLOTROPHIC YEAST ; HETEROLOGOUS PROTEIN ; YARROWIA-LIPOLYTICA ; PICHIA-PASTORIS ; METABOLIC-FLUX ; THERMOTOLERANT ; EXPRESSION ; PATHWAY ; THERMOMETHANOLICA ; CONSTRUCTION |
| 资助项目 | National Natural Science Foundation of China[31700082] ; National Natural Science Foundation of China[21808216] ; DMTO research grant from Dalian Institute of Chemicals Physics, CAS[DICP DMTO201701] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000463722500001 |
| 出版者 | BMC |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; DMTO research grant from Dalian Institute of Chemicals Physics, CAS ; DMTO research grant from Dalian Institute of Chemicals Physics, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DMTO research grant from Dalian Institute of Chemicals Physics, CAS ; DMTO research grant from Dalian Institute of Chemicals Physics, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DMTO research grant from Dalian Institute of Chemicals Physics, CAS ; DMTO research grant from Dalian Institute of Chemicals Physics, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DMTO research grant from Dalian Institute of Chemicals Physics, CAS ; DMTO research grant from Dalian Institute of Chemicals Physics, CAS |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/165686]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhou, Yongjin |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Peng,Gao, Jiaoqi,Zhou, Yongjin. CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications[J]. MICROBIAL CELL FACTORIES,2019,18:12. |
| APA | Cai, Peng,Gao, Jiaoqi,&Zhou, Yongjin.(2019).CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications.MICROBIAL CELL FACTORIES,18,12. |
| MLA | Cai, Peng,et al."CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications".MICROBIAL CELL FACTORIES 18(2019):12. |
入库方式： OAI收割
来源：大连化学物理研究所
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