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Multiplexed profiling of single-cell extracellular vesicles secretion

文献类型：期刊论文

作者Su, Haoran2,3; Ji, Yahui2,3; Qi, Dongyuan4; Li, Linmei3; Li, Xiaojie2; Luo, Yong5; Sun, Bo1; Zhang, Fuyin1; Lin, Bingcheng3; Liu, Tingjiao2
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版日期2019-03-26
卷号116期号:13页码:5979-5984
ISSN号0027-8424
关键词single-cell analysis extracellular vesicle cellular heterogeneity antibody barcodes
DOI10.1073/pnas.1814348116
通讯作者Liu, Tingjiao(tingjiao@dmu.edu.cn) ; Lu, Yao(luyao@dicp.ac.cn)
英文摘要Extracellular vesicles (EVs) are important intercellular mediators regulating health and diseases. Conventional methods for EV surface marker profiling, which was based on population measurements, masked the cell-to-cell heterogeneity in the quantity and phenotypes of EV secretion. Herein, by using spatially patterned antibody barcodes, we realized multiplexed profiling of single-cell EV secretion from more than 1,000 single cells simultaneously. Applying this platform to profile human oral squamous cell carcinoma (OSCC) cell lines led to a deep understanding of previously undifferentiated single-cell heterogeneity underlying EV secretion. Notably, we observed that the decrement of certain EV phenotypes (e.g., (CD63+) V) was associated with the invasive feature of both OSCC cell lines and primary OSCC cells. We also realized multiplexed detection of EV secretion and cytokines secretion simultaneously from the same single cells to investigate the multidimensional spectrum of cellular communications, from which we resolved tiered functional subgroups with distinct secretion profiles by visualized clustering and principal component analysis. In particular, we found that different cell subgroups dominated EV secretion and cytokine secretion. The technology introduced here enables a comprehensive evaluation of EV secretion heterogeneity at single-cell level, which may become an indispensable tool to complement current single-cell analysis and EV research.
WOS关键词CD63 TETRASPANIN ; EXOSOME SECRETION ; HETEROGENEITY ; REVEALS ; EXPRESSION ; MICROVESICLES ; CARCINOMA ; CYTOKINE ; RELEASE
资助项目National Natural Science Foundation of China[21874133] ; National Natural Science Foundation of China[21605143] ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant[2018217] ; Dalian Institute of Chemical Physics Grant[SZ201601]
WOS研究方向Science & Technology - Other Topics
语种英语
出版者NATL ACAD SCIENCES
WOS记录号WOS:000462382800029
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Youth Innovation Promotion Association Chinese Academy of Sciences Grant ; Dalian Institute of Chemical Physics Grant ; Dalian Institute of Chemical Physics Grant
源URL[http://cas-ir.dicp.ac.cn/handle/321008/165702]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Liu, Tingjiao; Lu, Yao
作者单位1.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China
2.Dalian Med Univ, Coll Stomatol, Dalian 116044, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
4.Dalian Med Univ, Affiliated Hosp 1, Dalian 116011, Peoples R China
5.Dalian Univ Technol, Dept Chem Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China
推荐引用方式
GB/T 7714		 Su, Haoran,Ji, Yahui,Qi, Dongyuan,et al. Multiplexed profiling of single-cell extracellular vesicles secretion[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(13):5979-5984.
APA Su, Haoran.,Ji, Yahui.,Qi, Dongyuan.,Li, Linmei.,Li, Xiaojie.,...&Lu, Yao.(2019).Multiplexed profiling of single-cell extracellular vesicles secretion.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(13),5979-5984.
MLA Su, Haoran,et al."Multiplexed profiling of single-cell extracellular vesicles secretion".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.13(2019):5979-5984.

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来源：大连化学物理研究所

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