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Dispersive Matrix Solid-Phase Extraction Method Coupled with High Performance Liquid Chromatography-Tandem Mass Spectrometry for Ultrasensitive Quantification of Endogenous Brassinosteroids in Minute Plants and Its Application for Geographical Distribution, Study

文献类型：期刊论文

作者Wang, Nan1,2; Li, Yuxuan1,2; Deng, Ting1,2; Duan, Chunfeng1; Ni, Lanxiu1,2; Guan, Yafeng1
刊名JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
出版日期2019-03-13
卷号67期号:10页码:3037-3045
关键词dispersive matrix solid-phase extraction endogenous brassinosteroids oilseed rape Arabidopsis thaliana seedlings geographical distribution
ISSN号0021-8561
DOI10.1021/acs.jafc.8b07224
通讯作者Duan, Chunfeng(cfduan@dicp.ac.cn) ; Guan, Yafeng(guanyafeng@dicp.ac.cn)
英文摘要An ultrasensitive analysis method for quantification of endogenous brassinosteroids in fresh minute plants was developed based on dispersive matrix solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry. During the dispersive matrix solid-phase extraction, plant samples were first ground with solid sorbent (dispersant) in one microcentrifuge tube and then centrifuged after adding extraction solvent and cleanup materials (another type of sorbent). Three protocols based on dispersive matrix solid-phase extraction were compared and discussed for plant samples with different matrix complexity. The choice of any protocol was a compromise of increasing purification efficiency and decreasing sample loss. Under optimized conditions, the limits of detection were 1.38-6.75 pg mL(-1) for five brassinosteroids in the oilseed rape samples. The intraday and interday precisions were in the range of 0.8%-9.8% and 4.6%-17.3%, respectively. The proposed method was successfully applied to detection of endogenous brassinosteroids in milligram oilseed rape (2.0 mg) and submilligram Arabidopsis thaliana seedlings (0.5 mg). Finally, the geographical distribution of five endogenous brassinosteroids of Brassica napus L. oilseed rape in different provinces of origin in the Yangtze River basin was described.
WOS关键词TRACE DETERMINATION ; SAMPLE PREPARATION ; MICROEXTRACTION ; SORBENT ; PHYTOHORMONES ; GIBBERELLINS ; RESIDUES ; HORMONES ; CADMIUM ; TISSUES
资助项目National Natural Science Foundation of China[21505135]
WOS研究方向Agriculture ; Chemistry ; Food Science & Technology
语种英语
WOS记录号WOS:000461538100033
出版者AMER CHEMICAL SOC
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://cas-ir.dicp.ac.cn/handle/321008/165810]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Duan, Chunfeng; Guan, Yafeng
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Nan,Li, Yuxuan,Deng, Ting,et al. Dispersive Matrix Solid-Phase Extraction Method Coupled with High Performance Liquid Chromatography-Tandem Mass Spectrometry for Ultrasensitive Quantification of Endogenous Brassinosteroids in Minute Plants and Its Application for Geographical Distribution, Study[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(10):3037-3045.
APA Wang, Nan,Li, Yuxuan,Deng, Ting,Duan, Chunfeng,Ni, Lanxiu,&Guan, Yafeng.(2019).Dispersive Matrix Solid-Phase Extraction Method Coupled with High Performance Liquid Chromatography-Tandem Mass Spectrometry for Ultrasensitive Quantification of Endogenous Brassinosteroids in Minute Plants and Its Application for Geographical Distribution, Study.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(10),3037-3045.
MLA Wang, Nan,et al."Dispersive Matrix Solid-Phase Extraction Method Coupled with High Performance Liquid Chromatography-Tandem Mass Spectrometry for Ultrasensitive Quantification of Endogenous Brassinosteroids in Minute Plants and Its Application for Geographical Distribution, Study".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.10(2019):3037-3045.

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来源：大连化学物理研究所

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