Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides
文献类型:期刊论文
作者 | Zhu, Xian-Yu1,2; Zhao, Yong1; Zhang, Huai-Dong1,3; Wang, Wen-Xia1; Cong, Hai-Hua2; Yin, Heng1 |
刊名 | MARINE DRUGS |
出版日期 | 2019-02-01 |
卷号 | 17期号:2页码:15 |
ISSN号 | 1660-3397 |
关键词 | chitin deacetylase deacetylation patterns chitooligosaccharides separating detecting |
DOI | 10.3390/md17020074 |
通讯作者 | Yin, Heng(yinheng@dicp.ac.cn) |
英文摘要 | Partially acetylated chitosan oligosaccharides (COS), which consists of N-acetylglucosamine (GlcNAc) and glucosamine (GlcN) residues, is a structurally complex biopolymer with a variety of biological activities. Therefore, it is challenging to elucidate acetylation patterns and the molecular structure-function relationship of COS. Herein, the detailed deacetylation pattern of chitin deacetylase from Saccharomyces cerevisiae, ScCDA(2), was studied. Which solves the randomization of acetylation patterns during COS produced by chemical. ScCDA(2) also exhibits about 8% and 20% deacetylation activity on crystalline chitin and colloid chitin, respectively. Besides, a method for separating and detecting partially acetylated chitosan oligosaccharides by high performance liquid chromatography and electrospray ionization mass spectrometry (HPLC-ESI-MS) system has been developed, which is fast and convenient, and can be monitored online. Mass spectrometry sequencing revealed that ScCDA(2) produced COS with specific acetylation patterns of DAAA, ADAA, AADA, DDAA, DADA, ADDA and DDDA, respectively. ScCDA(2) does not deacetylate the GlcNAc unit that is closest to the reducing end of the oligomer furthermore ScCDA(2) has a multiple-attack deacetylation mechanism on chitin oligosaccharides. This specific mode of action significantly enriches the existing limited library of chitin deacetylase deacetylation patterns. This fully defined COS may be used in the study of COS structure and function. |
WOS关键词 | CHITOOLIGOSACCHARIDES ; IDENTIFICATION ; PURIFICATION ; EFFICIENT ; POLYMERIZATION ; OLIGOMERS ; MECHANISM ; GLYCANS |
资助项目 | National Natural Science Foundation of China[31670803] ; National Natural Science Foundation of China[31770847] ; National Key Research and Development Project of China[2017YFD0200902] ; Dalian city Innovative Support Program for High-Level Talents[2015R010] ; CAS Youth Innovation Promotion Association[2015144] |
WOS研究方向 | Pharmacology & Pharmacy |
语种 | 英语 |
出版者 | MDPI |
WOS记录号 | WOS:000460795500002 |
资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Project of China ; National Key Research and Development Project of China ; Dalian city Innovative Support Program for High-Level Talents ; Dalian city Innovative Support Program for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Project of China ; National Key Research and Development Project of China ; Dalian city Innovative Support Program for High-Level Talents ; Dalian city Innovative Support Program for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Project of China ; National Key Research and Development Project of China ; Dalian city Innovative Support Program for High-Level Talents ; Dalian city Innovative Support Program for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Project of China ; National Key Research and Development Project of China ; Dalian city Innovative Support Program for High-Level Talents ; Dalian city Innovative Support Program for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/165828] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
通讯作者 | Yin, Heng |
作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China 2.Dalian Ocean Univ, Coll Food Sci & Engn, Dalian 116023, Peoples R China 3.Fujian Normal Univ, Coll Life Sci, Engn Res Ctr Ind Microbiol, Minist Educ, Fuzhou 350117, Fujian, Peoples R China |
推荐引用方式 GB/T 7714 | Zhu, Xian-Yu,Zhao, Yong,Zhang, Huai-Dong,et al. Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides[J]. MARINE DRUGS,2019,17(2):15. |
APA | Zhu, Xian-Yu,Zhao, Yong,Zhang, Huai-Dong,Wang, Wen-Xia,Cong, Hai-Hua,&Yin, Heng.(2019).Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides.MARINE DRUGS,17(2),15. |
MLA | Zhu, Xian-Yu,et al."Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides".MARINE DRUGS 17.2(2019):15. |
入库方式: OAI收割
来源:大连化学物理研究所
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