The study of the characteristics and hydrolysis properties of naringinase immobilized by porous silica material
文献类型：期刊论文
| 作者 | Zhang, Nan1; Luo, Jian1; Li, Qian1; Sun, Xitong2; Tian, Jing1; Fei, Xu1; Shi, Feng1; Liu, Xiangli1 |
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2019 |
| 卷号 | 9期号:8页码:4514-4520 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c9ra00075e |
| 通讯作者 | Li, Qian(liqian19820903@163.com) ; Sun, Xitong(sunxt@dicp.ac.cn) |
| 英文摘要 | Silica material has high specific surface area and excellent chemical stability, which make it useful for enzyme immobilization. In this work, naringinase was immobilized from fermentation broth of Aspergillus niger FFCC uv-11 by silica materials with different pore diameters of 2 nm (MCM-41), 7.7 nm (SBA-15) and 80 nm (silica gel). It was shown that SBA-15 had the highest naringinase activity, and this was chosen as a suitable carrier material for naringinase immobilization. First, SBA-15 was modified by glutaraldehyde at a concentration of 7% at 25 degrees C for 2 h, and it was then used for the immobilization of naringinase. At pH 3.5, the immobilized naringinase activity reached 467.62 U g(-1) at 40 degrees C for 4 h when the initial naringinase activity was 89.04 U mL(-1). Furthermore, at the optimal reaction temperature of 45 degrees C and pH of 4.5, the binding efficiency, activity recovery rate and specific activity of the immobilized naringinase were 63.66%, 87.64% and 517.43 U g(-1), respectively. Compared with free naringinase, in naringin hydrolysis, the immobilized naringinase performed over a wide pH application range and had good thermal stability. Even more important, the immobilized naringinase retained 61.81% of the residual naringinase activity after eight consecutive cycles, and kept 80.95% of the residual naringinase activity after one month of storage. This study provides an ideal carrier material and some basic data for naringinase immobilization technology, which will greatly promote the application of naringinase in industrial fruit juice processing. |
| WOS关键词 | CATALYTIC-ACTIVITY ; MESOPOROUS SILICA ; NANOPARTICLES |
| 资助项目 | National Natural Science Foundation of China[31601411] ; National Natural Science Foundation of China[21804129] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000459896700047 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/165868]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Li, Qian; Sun, Xitong |
| 作者单位 | 1.Dalian Polytech Univ, Dept Bioengn, Dalian 116034, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Nan,Luo, Jian,Li, Qian,et al. The study of the characteristics and hydrolysis properties of naringinase immobilized by porous silica material[J]. RSC ADVANCES,2019,9(8):4514-4520. |
| APA | Zhang, Nan.,Luo, Jian.,Li, Qian.,Sun, Xitong.,Tian, Jing.,...&Liu, Xiangli.(2019).The study of the characteristics and hydrolysis properties of naringinase immobilized by porous silica material.RSC ADVANCES,9(8),4514-4520. |
| MLA | Zhang, Nan,et al."The study of the characteristics and hydrolysis properties of naringinase immobilized by porous silica material".RSC ADVANCES 9.8(2019):4514-4520. |
入库方式： OAI收割
来源：大连化学物理研究所
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