Artificial Antibody with Site-Enhanced Multivalent Aptamers for Specific Capture of Circulating Tumor Cells
文献类型：期刊论文
| 作者 | Peng, Xiaojun; Zhang, Yukui2,3; Liu, Lukuan1,2,3; Yang, Kaiguang2; Gao, Hang1,2; Li, Xiao2; Chen, Yuanbo1,2; Zhang, Lihua2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2019-02-19 |
| 卷号 | 91期号:4页码:2591-2594 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.8b05259 |
| 通讯作者 | Zhang, Lihua(lihuazhang@dicp.ac.cn) |
| 英文摘要 | Isolation of circulating tumor cells (CTCs) from blood holds great potential to diagnose cancers and discover therapeutic targets. Herein, we reported a novel kind of artificial antibody, the cell-imprinted hydrogel with site-directed modification of aptamers (APT-CIH) to achieve the specific capture of CTCs. Cell-imprinted sites not only could be used to recognize target cells but also could be used as efficient scaffolds for assembling aptamers to enhance the capture efficiency and selectivity. Due to the synergistic effect of conformation recognition and multivalent interaction between the aptamers and target cells, APT-CIH showed high capture efficiency and selectivity to SMMC-7721 cells. In the coexistence of the 1000 times leukemia Jurkat cells, the enrichment factor of APT-CIH could reach as high as 21.6 +/- 3.1 toward target cells, while that relied only on cell imprinting or aptamer affinity was 8.1 +/- 5.0 or 10.1 +/- 1.3, respectively. Furthermore, the capture efficiency could reach 58.2% +/- 10.9% with 1000 SMMC-7721 cells spiked in 1 mL of blood. Moreover, 92% of the captured cells could be released, beneficial to carry out further biological and clinical study of CTCs. These results demonstrated that APT-CIH might have great potential in CTCs analysis. |
| WOS关键词 | CANCER-CELLS ; RELEASE ; RECOGNITION ; BINDING |
| 资助项目 | National Key Research and Development Program of China[2017YFA0505003] ; National Key Research and Development Program of China[2016YFA0501401] ; National Natural Science Foundation[21575143] ; National Natural Science Foundation[91543201] ; National Natural Science Foundation[21725506] ; CAS Key Project in Frontier Science[QYZDY-SSW-SLH017] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000459642400005 |
| 资助机构 | National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/165976]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Lihua |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Peng, Xiaojun,Zhang, Yukui,Liu, Lukuan,et al. Artificial Antibody with Site-Enhanced Multivalent Aptamers for Specific Capture of Circulating Tumor Cells[J]. ANALYTICAL CHEMISTRY,2019,91(4):2591-2594. |
| APA | Peng, Xiaojun.,Zhang, Yukui.,Liu, Lukuan.,Yang, Kaiguang.,Gao, Hang.,...&Zhang, Lihua.(2019).Artificial Antibody with Site-Enhanced Multivalent Aptamers for Specific Capture of Circulating Tumor Cells.ANALYTICAL CHEMISTRY,91(4),2591-2594. |
| MLA | Peng, Xiaojun,et al."Artificial Antibody with Site-Enhanced Multivalent Aptamers for Specific Capture of Circulating Tumor Cells".ANALYTICAL CHEMISTRY 91.4(2019):2591-2594. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。