Facile Fabrication of Biomimetic Chitosan Membrane with Honeycomb-Like Structure for Enrichment of Glycosylated Peptides
文献类型：期刊论文
| 作者 | Ou, Junjie3; Ye, Mingliang3; Zhang, Luwei2,3; Ma, Shujuan2,3; Chen, Yao1,3; Wang, Yan2,3; Uyama, Hiroshi2,4 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2019-02-19 |
| 卷号 | 91期号:4页码:2985-2993 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.8b05215 |
| 通讯作者 | Ou, Junjie(junjieou@dicp.ac.cn) ; Uyama, Hiroshi(uyama@chem.eng.osaka-u.ac.jp) |
| 英文摘要 | In the study of glycoproteomics with mass spectrometry, certain pretreatments of samples are required for eliminating the interference of nonglycopeptides and improving the efficiency of glycopeptides detection. Although hydrophilic interaction chromatography (HILIC) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups. In this work, a 1 mm thick biomimetic honeycomb chitosan membrane (BHCM) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for HILIC. The N-glycopeptides from trypsin digests of immunoglobulin G (IgG), mixture of IgG and bovine serum albumin (BSA), and serum proteins were enriched using this material and compared with a commercial material ZIC-HILIC. The biomimetic membrane could identify as many as 32 N-glycopeptides from the IgG digest, exhibiting high sensitivity (about 50 fmol) and a wide scope for glycopeptide enrichment. A molar ratio of IgG trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment. In addition, 270 unique N-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 mu L human serum. Furthermore, 48 unique O-glycosylation sites of 278 unique O-glycopeptides were identified from the triplicate analysis of 30 mu g deglycosylated fetuin digest. These results indicated that the chitosan-based membrane prepared in this work had great potential for pretreatment of samples in glycoproteomics. |
| WOS关键词 | MESOPOROUS SILICA MATERIALS ; HIGHLY SPECIFIC ENRICHMENT ; MASS-SPECTROMETRY ; EFFICIENT ENRICHMENT ; SELECTIVE ENRICHMENT ; N-GLYCOPEPTIDES ; GLYCOMICS ; IDENTIFICATION ; GLYCOPROTEINS ; COMPOSITES |
| 资助项目 | China State Key Basic Research Program Grant[2016YFA0501402] ; National Science Fund for Distinguished Young Scholars[21525524] ; National Natural Sciences Foundation of China[21575141] ; National Natural Sciences Foundation of China[21675125] ; National Natural Sciences Foundation of China[21606181] ; CAS-Weigao Research & Development Program[[2017]-009] ; Amygdalus pedunculata Engineering Technology Research Center of the State Forestry Administration ; Key Laboratory of Yulin Desert Plant Resources |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000459642400058 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | China State Key Basic Research Program Grant ; China State Key Basic Research Program Grant ; National Science Fund for Distinguished Young Scholars ; National Science Fund for Distinguished Young Scholars ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program ; Amygdalus pedunculata Engineering Technology Research Center of the State Forestry Administration ; Amygdalus pedunculata Engineering Technology Research Center of the State Forestry Administration ; Key Laboratory of Yulin Desert Plant Resources ; Key Laboratory of Yulin Desert Plant Resources ; China State Key Basic Research Program Grant ; China State Key Basic Research Program Grant ; National Science Fund for Distinguished Young Scholars ; National Science Fund for Distinguished Young Scholars ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program ; Amygdalus pedunculata Engineering Technology Research Center of the State Forestry Administration ; Amygdalus pedunculata Engineering Technology Research Center of the State Forestry Administration ; Key Laboratory of Yulin Desert Plant Resources ; Key Laboratory of Yulin Desert Plant Resources ; China State Key Basic Research Program Grant ; China State Key Basic Research Program Grant ; National Science Fund for Distinguished Young Scholars ; National Science Fund for Distinguished Young Scholars ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program ; Amygdalus pedunculata Engineering Technology Research Center of the State Forestry Administration ; Amygdalus pedunculata Engineering Technology Research Center of the State Forestry Administration ; Key Laboratory of Yulin Desert Plant Resources ; Key Laboratory of Yulin Desert Plant Resources ; China State Key Basic Research Program Grant ; China State Key Basic Research Program Grant ; National Science Fund for Distinguished Young Scholars ; National Science Fund for Distinguished Young Scholars ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program ; Amygdalus pedunculata Engineering Technology Research Center of the State Forestry Administration ; Amygdalus pedunculata Engineering Technology Research Center of the State Forestry Administration ; Key Laboratory of Yulin Desert Plant Resources ; Key Laboratory of Yulin Desert Plant Resources |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/165978]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Ou, Junjie; Uyama, Hiroshi |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Northwest Univ, Key Lab Synthet & Nat Funct Mol Chem, Natl Demonstrat Ctr Expt Chem Educ, Minist Educ,Coll Chem & Mat Sci, Xian 710127, Shaanxi, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 4.Osaka Univ, Grad Sch Engn, Dept Appl Chem, Suita, Osaka 5650871, Japan |
| 推荐引用方式 GB/T 7714 | Ou, Junjie,Ye, Mingliang,Zhang, Luwei,et al. Facile Fabrication of Biomimetic Chitosan Membrane with Honeycomb-Like Structure for Enrichment of Glycosylated Peptides[J]. ANALYTICAL CHEMISTRY,2019,91(4):2985-2993. |
| APA | Ou, Junjie.,Ye, Mingliang.,Zhang, Luwei.,Ma, Shujuan.,Chen, Yao.,...&Uyama, Hiroshi.(2019).Facile Fabrication of Biomimetic Chitosan Membrane with Honeycomb-Like Structure for Enrichment of Glycosylated Peptides.ANALYTICAL CHEMISTRY,91(4),2985-2993. |
| MLA | Ou, Junjie,et al."Facile Fabrication of Biomimetic Chitosan Membrane with Honeycomb-Like Structure for Enrichment of Glycosylated Peptides".ANALYTICAL CHEMISTRY 91.4(2019):2985-2993. |
入库方式： OAI收割
来源：大连化学物理研究所
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