Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization
文献类型：期刊论文
| 作者 | Feng, Yingang1,2; Cui, Qiu1,2; Liu, Shiyue1,2,3; Liu, Ya-Jun1,2; Li, Bin1,2 |
| 刊名 | BIOTECHNOLOGY FOR BIOFUELS
 |
| 出版日期 | 2019-02-18 |
| 卷号 | 12页码:12 |
| ISSN号 | 1754-6834 |
| 关键词 | Cellulase Cellulosome Clostridium thermocellum Fermentable sugar Lignocellulose Saccharification |
| DOI | 10.1186/s13068-019-1374-2 |
| 通讯作者 | Cui, Qiu(cuiqiu@qibebt.ac.cn) |
| 英文摘要 | BackgroundThe industrial conversion of biomass to high-value biofuels and biochemical is mainly restricted by lignocellulose solubilization. Consolidated bio-saccharification (CBS) is considered a promising process for lignocellulose solubilization depending on whole-cell biocatalysts that simultaneously perform effective cellulase production and hydrolysis. However, it usually takes a long time to reach a high saccharification level using the current CBS biocatalyst and process.ResultsTo promote the saccharification efficiency and reduce the cost, a Clostridium thermocellum recombinant strain pyrF::KBm was constructed as a new CBS biocatalyst in this study. The key CBS factors, including the medium, inoculum size and cultivation, and substrate load, were investigated and optimized. The saccharification process was also stimulated by adding free hemicellulases, suggesting the need to further enhance hemicellulase activity of the whole-cell catalyst. Under the optimal conditions, the CBS process was shortened by 50% with pretreated wheat straw as the substrate. The sugar yield reached 0.795g/g and the saccharification level was 89.3%.ConclusionsThis work provided a new biocatalyst and an optimized process of CBS and confirmed that CBS is a feasible strategy for cost-efficient solubilization of lignocellulose, which will greatly promote the industrial utilization of lignocellulosic biomass. |
| WOS关键词 | CLOSTRIDIUM-THERMOCELLUM ; ETHANOL ; CELLULOSE ; GLUCOSE ; BIOMASS ; GROWTH ; YIELD |
| 资助项目 | Transformational Technologies for Clean Energy and Demonstration, Strategic Priority Research Program of the Chinese Academy of Sciences[XDA 21060201] ; National Natural Science Foundation of China[31470210] ; National Natural Science Foundation of China[31570029] ; National Natural Science Foundation of China[31670735] ; National Natural Science Foundation of China[31870568] ; National Natural Science Foundation of China[31661143023] ; Key Technology Research and Development Program of Shandong[2018GSF1160165] ; Major Program of Shandong Provincial Natural Science Foundation[ZR2018ZB0208] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Energy & Fuels |
| 语种 | 英语 |
| 出版者 | BMC |
| WOS记录号 | WOS:000459152400002 |
| 资助机构 | Transformational Technologies for Clean Energy and Demonstration, Strategic Priority Research Program of the Chinese Academy of Sciences ; Transformational Technologies for Clean Energy and Demonstration, Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Technology Research and Development Program of Shandong ; Key Technology Research and Development Program of Shandong ; Major Program of Shandong Provincial Natural Science Foundation ; Major Program of Shandong Provincial Natural Science Foundation ; Transformational Technologies for Clean Energy and Demonstration, Strategic Priority Research Program of the Chinese Academy of Sciences ; Transformational Technologies for Clean Energy and Demonstration, Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Technology Research and Development Program of Shandong ; Key Technology Research and Development Program of Shandong ; Major Program of Shandong Provincial Natural Science Foundation ; Major Program of Shandong Provincial Natural Science Foundation ; Transformational Technologies for Clean Energy and Demonstration, Strategic Priority Research Program of the Chinese Academy of Sciences ; Transformational Technologies for Clean Energy and Demonstration, Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Technology Research and Development Program of Shandong ; Key Technology Research and Development Program of Shandong ; Major Program of Shandong Provincial Natural Science Foundation ; Major Program of Shandong Provincial Natural Science Foundation ; Transformational Technologies for Clean Energy and Demonstration, Strategic Priority Research Program of the Chinese Academy of Sciences ; Transformational Technologies for Clean Energy and Demonstration, Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Key Technology Research and Development Program of Shandong ; Key Technology Research and Development Program of Shandong ; Major Program of Shandong Provincial Natural Science Foundation ; Major Program of Shandong Provincial Natural Science Foundation |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/165990]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Cui, Qiu |
| 作者单位 | 1.Chinese Acad Sci, Dalian Natl Lab Clean Energy, Dalian, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Yingang,Cui, Qiu,Liu, Shiyue,et al. Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization[J]. BIOTECHNOLOGY FOR BIOFUELS,2019,12:12. |
| APA | Feng, Yingang,Cui, Qiu,Liu, Shiyue,Liu, Ya-Jun,&Li, Bin.(2019).Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization.BIOTECHNOLOGY FOR BIOFUELS,12,12. |
| MLA | Feng, Yingang,et al."Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization".BIOTECHNOLOGY FOR BIOFUELS 12(2019):12. |
入库方式： OAI收割
来源：大连化学物理研究所
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