Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of His-tagged protein
文献类型：期刊论文
| 作者 | Qin, Qian1,2; Wang, Bohong1,2; Xu, Guowang1; Xia, Tian1; Lv, Wangjie1,2; Sun, Xiaoshan1,2; Chang, Mengmeng1,2; Shi, Xianzhe1 |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2019-02-01 |
| 卷号 | 42期号:3页码:744-753 |
| ISSN号 | 1615-9306 |
| 关键词 | immobilized metal-ion affinity chromatography layer-by-layer assembly magnetic nanoparticles protein purification |
| DOI | 10.1002/jssc.201800969 |
| 通讯作者 | Shi, Xianzhe(shixianzhe@dicp.ac.cn) |
| 英文摘要 | Employing immobilized metal-ion affinity chromatography and magnetic separation could ideally provide a useful analytical strategy for purifying His-tagged protein. In the current study, a facile route was designed to prepare CMPEI-Ni2+@SiO2@Fe3O4 (CMPEI=carboxymethylated polyethyleneimine) magnetic nanoparticles composed of a strong magnetic core of Fe3O4 and a Ni2+-immobilized carboxymethylated polyethyleneimine coated outside shell, which was formed by electrostatic interactions between polyanionic electrolyte of carboxymethylated polyethyleneimine and positively charged surface of 3-(trimethoxysilyl) propylamin modified SiO2@Fe3O4. The resulting CMPEI-Ni2+@SiO2@Fe3O4 composite nanoparticles displayed well-uniform structure and high magnetic responsiveness. Hexa His-tagged peptides and purified His-tagged recombinant retinoid X receptor alpha were chosen as the model samples to evaluate the adsorption, capacity, and reusability of the composite nanoparticles. The results demonstrated the CMPEI-Ni2+@SiO2@Fe3O4 nanoparticles possessed rapid adsorption, large capacity, and good recyclability. The obtained nanoparticles were further used to purify His-tagged protein in practical environment. It was found that the nanoparticles could selectively capture His-tagged recombinant retinoid X receptor protein from complex cell lysate. Owing to its easy synthesis, large binding capacity, and good reusability, the prepared CMPEI-Ni2+@SiO2@Fe3O4 magnetic nanoparticles have great potential for application in biotechnological fields. |
| WOS关键词 | SELECTIVE REMOVAL ; SEPARATION ; ADSORPTION ; EXPRESSION ; IMMOBILIZATION ; MICROSPHERES ; EXTRACTION ; COMPOSITE ; SILICA |
| 资助项目 | National Natural Science Foundation of China[21575142] ; National Natural Science Foundation of China[21705147] ; National Natural Science Foundation of China[21435006] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000457651000013 |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/166094]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Shi, Xianzhe |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Qian,Wang, Bohong,Xu, Guowang,et al. Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of His-tagged protein[J]. JOURNAL OF SEPARATION SCIENCE,2019,42(3):744-753. |
| APA | Qin, Qian.,Wang, Bohong.,Xu, Guowang.,Xia, Tian.,Lv, Wangjie.,...&Shi, Xianzhe.(2019).Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of His-tagged protein.JOURNAL OF SEPARATION SCIENCE,42(3),744-753. |
| MLA | Qin, Qian,et al."Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of His-tagged protein".JOURNAL OF SEPARATION SCIENCE 42.3(2019):744-753. |
入库方式： OAI收割
来源：大连化学物理研究所
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