Modified metabolites mapping by liquid chromatography-high resolution mass spectrometry using full scan/all ion Cheek for fragmentation/neutral loss acquisition
文献类型：期刊论文
| 作者 | He, Jun1; Xu, Guowang2; Qin, Qian2,4; Li, Hua3; Shi, Xianzhe2 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2019-01-04 |
| 卷号 | 1583页码:80-87 |
| 关键词 | Modified metabolites All ion fragmentation Neutral loss Nontargeted analysis Metabolomics |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2018.11.014 |
| 通讯作者 | Shi, Xianzhe(shixianzhe@dicp.ac.cn) |
| 英文摘要 | Modified metabolites play important roles in diagnostic monitoring, oxidative response and physiological regulation. Comprehensive analytical methods are greatly needed for improving the coverage of modified metabolites and studying their physiological function. Here, a novel nontargeted profiling method for mapping modified metabolites was developed by liquid chromatography-high resolution mass spectrometry with full scan/all ion fragmentation/neutral loss (FS/AIF/NL) data acquisition. Modified metabolites were unbiasedly defined with less false positive results by mixed standards verification. Thirteen types of modified metabolites in urine were analyzed at the same time, and 198 of 307 modified metabolites at positive mode and 166 of 366 modified metabolites at negative mode were putatively identified. The modified metabolites profiling method was applied for investigating the metabolic differences between the patients with breast cancer and health controls. As a result, many modified metabolites with glu-curonidation, ribosylation and indole acetylation modification were significantly up-regulated in breast cancer and four modified metabolites including 7-methylguanosine, N-4-acetylcytidine, dihyroxy-1H-indole glucuronide I and indole-3-acetic acid-O-glucuronide were identified as potential biomarkers for the diagnose of breast cancer. (C) 2018 Elsevier B.V. All rights reserved. |
| WOS关键词 | MODIFICATION-SPECIFIC METABOLOMICS ; MODIFIED NUCLEOSIDES ; IDENTIFICATION ; PROTEOMICS |
| 资助项目 | National Natural Science Foundation of China[21575142] ; National Natural Science Foundation of China[21505132] ; National Natural Science Foundation of China[21705147] ; National Natural Science Foundation of China[21435006] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000455692100010 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/166198]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Shi, Xianzhe |
| 作者单位 | 1.Shekou Peoples Hosp, Dept clin lab, Shenzhen 518052, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Southern Univ Sci & Technol, Mat Characterizat & Preparat Ctr, Shenzhen 518055, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Jun,Xu, Guowang,Qin, Qian,et al. Modified metabolites mapping by liquid chromatography-high resolution mass spectrometry using full scan/all ion Cheek for fragmentation/neutral loss acquisition[J]. JOURNAL OF CHROMATOGRAPHY A,2019,1583:80-87. |
| APA | He, Jun,Xu, Guowang,Qin, Qian,Li, Hua,&Shi, Xianzhe.(2019).Modified metabolites mapping by liquid chromatography-high resolution mass spectrometry using full scan/all ion Cheek for fragmentation/neutral loss acquisition.JOURNAL OF CHROMATOGRAPHY A,1583,80-87. |
| MLA | He, Jun,et al."Modified metabolites mapping by liquid chromatography-high resolution mass spectrometry using full scan/all ion Cheek for fragmentation/neutral loss acquisition".JOURNAL OF CHROMATOGRAPHY A 1583(2019):80-87. |
入库方式： OAI收割
来源：大连化学物理研究所
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