In-situ Proteomic Characterization of Protein Conformation on Nanoparticle Surface by Cross-linking Mass Spectrometry
文献类型：期刊论文
| 作者 | Zhang Hong-Yan1,2; Wu Ren-An1; Zhou Ye1,2 |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018-10-01 |
| 卷号 | 46期号:10页码:1570-1580 |
| 关键词 | Chemical cross-linking mass spectrometry Proteomics Nanoparticle Protein conformation |
| ISSN号 | 0253-3820 |
| DOI | 10.11895/j.issn.0253-3820.181290 |
| 通讯作者 | Wu Ren-An(wurenan@dicp.ac.cn) |
| 英文摘要 | The conformation change of bovine serum albumin (BSA) absorbed on Fe3O4 nanoparticle surface was characterized by chemical cross-linking mass spectrometry (CXMS)-based proteomics approach. The effects of nanoparticle surface curvatures and initial protein concentrations on protein conformation change were investigated by the CXMS. The results revealed that the BSA adsorbed on the surface of Fe3O4 nanoparticle could undergo an altered conformational state in comparison to that of BSA in solution as the crosslinking frequency of some amino acid residues of the BSA varied significantly, with the assistant analysis of the attenuated total reflectance Fourier transfer infrared spectroscopy (ATR-FTIR). By virtue of the high-throughput, high accuracy and high sensitivity of mass spectrometry, the CXMS-based proteomic method could provide structural information of proteins adsorbed on nanoparticle surface by crosslinking restraints and would be promised to be applied to the analysis of nano-bio interaction for proteins in complex biological systems. |
| WOS关键词 | ALBUMIN ADSORPTION ; COMPLEXES |
| 资助项目 | National Natural Science Foundation of China[21375125] ; National Natural Science Foundation of China[21675156] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000455914000004 |
| 出版者 | ELSEVIER SCIENCE INC |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/166218]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Wu Ren-An |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Hong-Yan,Wu Ren-An,Zhou Ye. In-situ Proteomic Characterization of Protein Conformation on Nanoparticle Surface by Cross-linking Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2018,46(10):1570-1580. |
| APA | Zhang Hong-Yan,Wu Ren-An,&Zhou Ye.(2018).In-situ Proteomic Characterization of Protein Conformation on Nanoparticle Surface by Cross-linking Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,46(10),1570-1580. |
| MLA | Zhang Hong-Yan,et al."In-situ Proteomic Characterization of Protein Conformation on Nanoparticle Surface by Cross-linking Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 46.10(2018):1570-1580. |
入库方式： OAI收割
来源：大连化学物理研究所
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