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Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants

文献类型：期刊论文

作者Zhou, Han2; Wang, Chaoran2; Du, Nana1,2; Si, Wei2; Liu, Yanfang2; Guo, Zhimou2; Zhou, Weijia1,2; Liang, Xinmiao2
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
出版日期2019-01-15
卷号1105页码:148-155
关键词Alkaloid enrichment Electrostatic repulsion Reversed-phase/strong anion-exchange Scopolia tangutica Mixed-mode stationary phase Class separation
ISSN号1570-0232
DOI10.1016/j.jchromb.2018.12.024
通讯作者Liu, Yanfang(liuyanfang@dicp.ac.cn) ; Liang, Xinmiao(liangxm@dicp.ac.cn)
英文摘要Since the content of alkaloids is usually low in plants and they are easily co-eluted with other constituents, enrichment of alkaloids is essential in the discovery of bioactive lead compounds from natural products. In this paper, an easy SPE enrichment method was developed in a buffer-free solvent system based on electrostatic repulsion mechanism. The feasibility of the new method was verified by successful enrichment of alkaloids from Scopolia tangutica (S. tangutica) with an optimized eluting condition. Then this developed method was applied to other representative plants in different families, including Przewalskia tangutica and Peganum harmala L, Lycoris radiata and Menispermum dauricum DC, which enlarged the scope of applicability. Additionally, the new SPE procedure avoided possible structural change destruction caused by pH change. Simple solvent system, including formic acid (FA) and methanol, would benefit subsequent mass analysis, quantity determination and bioactivity screening, and so on.
WOS关键词LIQUID-CHROMATOGRAPHY ; ACONITUM ALKALOIDS ; ALZHEIMERS-DISEASE ; PHASE ; EXTRACTION ; SEPARATION ; SURFACE ; COLUMN
资助项目State Key Program of National Natural Science Foundation of China[U1508221] ; National Science Foundation of China[21405155] ; National Science Foundation of China[31670374] ; DICP-CMC innovation institute of medicine
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000456893100017
出版者ELSEVIER SCIENCE BV
资助机构State Key Program of National Natural Science Foundation of China ; State Key Program of National Natural Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; DICP-CMC innovation institute of medicine ; DICP-CMC innovation institute of medicine ; State Key Program of National Natural Science Foundation of China ; State Key Program of National Natural Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; DICP-CMC innovation institute of medicine ; DICP-CMC innovation institute of medicine ; State Key Program of National Natural Science Foundation of China ; State Key Program of National Natural Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; DICP-CMC innovation institute of medicine ; DICP-CMC innovation institute of medicine ; State Key Program of National Natural Science Foundation of China ; State Key Program of National Natural Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; DICP-CMC innovation institute of medicine ; DICP-CMC innovation institute of medicine
源URL[http://cas-ir.dicp.ac.cn/handle/321008/166220]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Liu, Yanfang; Liang, Xinmiao
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Han,Wang, Chaoran,Du, Nana,et al. Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2019,1105:148-155.
APA Zhou, Han.,Wang, Chaoran.,Du, Nana.,Si, Wei.,Liu, Yanfang.,...&Liang, Xinmiao.(2019).Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1105,148-155.
MLA Zhou, Han,et al."Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1105(2019):148-155.

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来源：大连化学物理研究所

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