Efficient co-expression of multiple enzymes from a single promoter mediated by virus 2A sequence in the oleaginous yeast Rhodosporidium toruloides
文献类型：期刊论文
| 作者 | Yang, Xiaobing1; Wang, Qian1; Zhao, Zongbao Kent1; Zhang, Sufang1; Jiao, Xiang1,2; Zhang, Qi1,2 |
| 刊名 | FEMS YEAST RESEARCH
 |
| 出版日期 | 2018-12-01 |
| 卷号 | 18期号:8页码:8 |
| 关键词 | red yeasts Rhodosporidium toruloides 2A sequence gene co-expression beta-carotenoids |
| ISSN号 | 1567-1356 |
| DOI | 10.1093/femsyr/foy086 |
| 通讯作者 | Zhao, Zongbao Kent(zhaozb@dicp.ac.cn) |
| 英文摘要 | The red yeast Rhodosporidium toruloide is a versatile host for production of lipids and carotenoids. Genetic tools are underdeveloped for red yeasts due to their unique genetics and physiology. Currently expression of a heterogonous gene in red yeasts is largely based on integration of the designed cassette by Agrobacterium mediated transformation, yet this method is somewhat restricted when multiple genes are required to be expressed due to the lack of functional genetic elements. Here we demonstrate that virus 2A sequence is effective to mediate co-expression of multiple enzymes in R. toruloides. Two different 2A sequences, Porcine teschovirus-1 2A (P2A) and foot-and-mouth disease virus 2A (F2A), were evaluated. It was found that P2A sequence was more effective for co-expression of two antibiotic selection markers. Co-expression of three antibiotic resistance proteins was successful from a single promoter mediated by P2A sequence. When three heterogeneous enzymes responsible for beta-carotene biosynthesis were co-expressed, recombinant R. toruloides strains produced up to 4.5-fold more beta-carotene than that of the parental one. The use of 2A sequence can facilitate cassette construction to engineer advanced cell factories for production of lipids and related oleochemicals. |
| WOS关键词 | LIPID PRODUCTION ; RF CLONING ; GLYCEROL ; TRANSFORMATION ; CAROTENOIDS ; PRODUCTS ; GENES ; Y4 |
| 资助项目 | National Natural Science Foundation of China[51561145014] ; National Natural Science Foundation of China[51761145014] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology ; Mycology |
| 语种 | 英语 |
| WOS记录号 | WOS:000456030700005 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/166224]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhao, Zongbao Kent |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, 19 Yuquan Lu, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Xiaobing,Wang, Qian,Zhao, Zongbao Kent,et al. Efficient co-expression of multiple enzymes from a single promoter mediated by virus 2A sequence in the oleaginous yeast Rhodosporidium toruloides[J]. FEMS YEAST RESEARCH,2018,18(8):8. |
| APA | Yang, Xiaobing,Wang, Qian,Zhao, Zongbao Kent,Zhang, Sufang,Jiao, Xiang,&Zhang, Qi.(2018).Efficient co-expression of multiple enzymes from a single promoter mediated by virus 2A sequence in the oleaginous yeast Rhodosporidium toruloides.FEMS YEAST RESEARCH,18(8),8. |
| MLA | Yang, Xiaobing,et al."Efficient co-expression of multiple enzymes from a single promoter mediated by virus 2A sequence in the oleaginous yeast Rhodosporidium toruloides".FEMS YEAST RESEARCH 18.8(2018):8. |
入库方式： OAI收割
来源：大连化学物理研究所
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