A disposable microcapsule array chip fabricated by ice printing combined with isothermal amplification for Salmonella DNA detection
文献类型：期刊论文
| 作者 | Guo, Dan2; Zhang, Xinxiang1; Zhou, Yinglin1; Li, Zhihong3,4; He, Enqi2,4; Cao, Ting1; Cai, Liangyuan1 |
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2018 |
| 卷号 | 8期号:69页码:39561-39566 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c8ra07045h |
| 通讯作者 | Zhou, Yinglin(zhouyl@pku.edu.cn) ; Zhang, Xinxiang(zxx@pku.edu.cn) ; Li, Zhihong(zhhli@pku.edu.cn) |
| 英文摘要 | In this work, a novel microcapsule array chip was fabricated for the detection of Salmonella DNA by integrating an ice-printing technique with DNA isothermal amplification. Reaction solutions were previously sealed in the microcapsule array chip via ice printing. To protect the relatively fragile DNA isothermal amplification system, an extra polystyrene (PS) film was introduced to isolate the reaction solution from photopolymer precursor, which was proved to be a vital step for providing a clean and stable environment for DNA amplification reaction. Detection operation can be done by simply injecting sample DNA into the microcapsule by an easily accessible syringe, and the result can be directly obtained through color change within 90 minutes. This method shows good sensitivity, specificity and stability. |
| WOS关键词 | MICROFLUIDIC DEVICE ; POINT ; LAMP ; EXTRACTION ; BIOSENSOR ; ASSAY |
| 资助项目 | National Key Research and Development Program of China[2016YFA0200802] ; National Natural Science Foundation of China[21675004] ; National Natural Science Foundation of China[21575005] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000452116300037 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/166414]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Xinxiang; Zhou, Yinglin; Li, Zhihong |
| 作者单位 | 1.Peking Univ, Coll Chem, Key Lab Bioorgan Chem & Mol Engn, BNLMS,Minist Educ, Beijing 100871, Peoples R China 2.Tsinghua Univ, State Key Lab Tribol, Dept Mech Engn, Beijing 100084, Peoples R China 3.Peking Univ, Inst Microelect, Natl Key Lab Sci & Technol Micro Nano Fabricat, Beijing 100871, Peoples R China 4.Tsinghua Univ, Ctr Nano & Micro Mech, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Dan,Zhang, Xinxiang,Zhou, Yinglin,et al. A disposable microcapsule array chip fabricated by ice printing combined with isothermal amplification for Salmonella DNA detection[J]. RSC ADVANCES,2018,8(69):39561-39566. |
| APA | Guo, Dan.,Zhang, Xinxiang.,Zhou, Yinglin.,Li, Zhihong.,He, Enqi.,...&Cai, Liangyuan.(2018).A disposable microcapsule array chip fabricated by ice printing combined with isothermal amplification for Salmonella DNA detection.RSC ADVANCES,8(69),39561-39566. |
| MLA | Guo, Dan,et al."A disposable microcapsule array chip fabricated by ice printing combined with isothermal amplification for Salmonella DNA detection".RSC ADVANCES 8.69(2018):39561-39566. |
入库方式： OAI收割
来源：大连化学物理研究所
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