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Identification of Alkaloids from Corydalis yanhusuo W. T. Wang as Dopamine D-1 Receptor Antagonists by Using CRE-Luciferase Reporter Gene Assay

文献类型：期刊论文

作者Wu, Lehao1; Zhang, Weiyue1; Qiu, Xin1; Wang, Chaoran2; Liu, Yanfang2; Wang, Zhiwei3; Yu, Yang1; Ye, Richard D.1,4; Zhang, Yan1
刊名MOLECULES
出版日期2018-10-01
卷号23期号:10页码:10
关键词Corydalis yanhusuoW. T. Wang luciferase reporter gene assay D-1 receptor FLIPR
ISSN号1420-3049
DOI10.3390/molecules23102585
通讯作者Zhang, Yan(zhangyan_sjtu@sjtu.edu.cn)
英文摘要Corydalis yanhusuo W. T. Wang (C. yanhusuo) has been traditionally used for drug addiction and pain relief in China. In our previous study, we showed that the extract of C. yanhusuo blocks dopamine receptors, demonstrating that its pharmacological activities are mostly due to the antagonistic effects of some of its components at dopamine receptors. As part of our ongoing project on C. yanhusuo, the aim of the present study is to establish a high-throughput and low-cost screening assay system and test the abilities of the isolated alkaloids from C. yanhusuo to inhibit dopamine-induced dopamine D-1 receptor activity. By using our established cyclic adenosine monophosphate (cAMP)-response element (CRE)-luciferase reporter gene assay system, we identified eight alkaloids from C. yanhusuo with D-1 receptor antagonistic activities. We next validated the activities of these compounds using fluorometric imaging plate reader (FLIPR) assay by measuring the intracellular Ca2+ change. Six out of eight compounds, including tetrahydropalmatine, corydaline, 13-methyldehydrocorydalmine, dehydrocorybubine, dehydrocorydaline, and columbamine, can be confirmed for their inhibitory activities. The dopamine-receptor-antagonistic effects of four compounds, including 13-methyldehydrocorydalmine, dehydrocorydaline, columbamine, and corydaline, are reported for the first time. The present study provides an important pharmacological basis to support the traditional use of C. yanhusuo in China.
WOS关键词QUATERNARY ALKALOIDS ; DEHYDROCORYDALINE ; ANTINOCICEPTION ; PURIFICATION ; ACTIVATION
资助项目National Science Foundation of China[81402806] ; National Science Foundation of China[31470865] ; Science and Technology Development Fund of Macau[FDCT 072/2015/A2] ; University of Macau[CPG2015-00018-ICMS]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000451201400181
出版者MDPI
资助机构National Science Foundation of China ; National Science Foundation of China ; Science and Technology Development Fund of Macau ; Science and Technology Development Fund of Macau ; University of Macau ; University of Macau ; National Science Foundation of China ; National Science Foundation of China ; Science and Technology Development Fund of Macau ; Science and Technology Development Fund of Macau ; University of Macau ; University of Macau ; National Science Foundation of China ; National Science Foundation of China ; Science and Technology Development Fund of Macau ; Science and Technology Development Fund of Macau ; University of Macau ; University of Macau ; National Science Foundation of China ; National Science Foundation of China ; Science and Technology Development Fund of Macau ; Science and Technology Development Fund of Macau ; University of Macau ; University of Macau
源URL[http://cas-ir.dicp.ac.cn/handle/321008/166562]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Zhang, Yan
作者单位1.Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.DICP CMC Innovat Inst Med, Taizhou 225300, Peoples R China
4.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau 999078, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Lehao,Zhang, Weiyue,Qiu, Xin,et al. Identification of Alkaloids from Corydalis yanhusuo W. T. Wang as Dopamine D-1 Receptor Antagonists by Using CRE-Luciferase Reporter Gene Assay[J]. MOLECULES,2018,23(10):10.
APA Wu, Lehao.,Zhang, Weiyue.,Qiu, Xin.,Wang, Chaoran.,Liu, Yanfang.,...&Zhang, Yan.(2018).Identification of Alkaloids from Corydalis yanhusuo W. T. Wang as Dopamine D-1 Receptor Antagonists by Using CRE-Luciferase Reporter Gene Assay.MOLECULES,23(10),10.
MLA Wu, Lehao,et al."Identification of Alkaloids from Corydalis yanhusuo W. T. Wang as Dopamine D-1 Receptor Antagonists by Using CRE-Luciferase Reporter Gene Assay".MOLECULES 23.10(2018):10.

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来源：大连化学物理研究所

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