mRNAs are sorted for export or degradation before passing through nuclear speckles
文献类型：期刊论文
| 作者 | Fan, Jing1; Kuai, Bin1; Wang, Ke1; Wang, Lantian1; Wang, Yimin1; Wu, Xudong2; Chi, Binkai1; Li, Guohui2; Cheng, Hong1 |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2018-09-19 |
| 卷号 | 46期号:16页码:8404-8416 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gky650 |
| 通讯作者 | Cheng, Hong(hcheng@sibcb.ac.cn) |
| 英文摘要 | A significant fraction of mRNAs are degraded by the nuclear exosome in normal cells. Here, we studied where and when these exosome target mRNAs are sorted away from properly exported ones in the cells. We show that upon exosome inactivation, polyA RNAs are apparently accumulated in nuclear foci that are distinct from nuclear speckles (NSs), and provide several lines of evidence supporting that these polyA RNAs mainly correspond to accumulating exosome target mRNAs. These results suggest that exosomal mRNA degradationmostly occurs outside of NSs. In support of this possibility, targeting exosome target mRNAs to NSs stabilizes them by preventing exosomal degradation. Furthermore, inhibiting mRNA release from NSs does not attenuate exosomal degradation in normal cells, and results in polyA RNA accumulation both inside and outside of NSs in exosome inactivated cells, suggesting that passage through NSs is not required for sorting mRNAs for degradation or export. Indeed, exosome target mRNAs that normally do not enter NSs are exported upon exosome inactivation. Together, our data suggest that exosome target mRNAs are mainly degraded in the nucleoplasm before entering NSs and rapid removal of these mRNAs is important for preventing their nuclear export. |
| WOS关键词 | HUMAN TREX COMPLEX ; POLY(A) RNA ; EXOSOME ; PROTEIN ; RECRUITMENT ; ALYREF ; END |
| 资助项目 | National Key R&D Program of China[2017YFA0504400] ; National Natural Science Foundation of China[21625302] ; National Natural Science Foundation of China[31570822] ; National Natural Science Foundation of China[91540104] ; National Natural Science Foundation of China[31770880] ; China Postdoctoral Science Foundation[BX20180333] ; China Postdoctoral Science Foundation[2018M630481] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000450950500033 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/166572]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Cheng, Hong |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Mol Biol, Shanghai Key Lab Mol Androl,Univ Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci,Shanghai Inst Bio, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Mol Modeling & Design, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Jing,Kuai, Bin,Wang, Ke,et al. mRNAs are sorted for export or degradation before passing through nuclear speckles[J]. NUCLEIC ACIDS RESEARCH,2018,46(16):8404-8416. |
| APA | Fan, Jing.,Kuai, Bin.,Wang, Ke.,Wang, Lantian.,Wang, Yimin.,...&Cheng, Hong.(2018).mRNAs are sorted for export or degradation before passing through nuclear speckles.NUCLEIC ACIDS RESEARCH,46(16),8404-8416. |
| MLA | Fan, Jing,et al."mRNAs are sorted for export or degradation before passing through nuclear speckles".NUCLEIC ACIDS RESEARCH 46.16(2018):8404-8416. |
入库方式： OAI收割
来源：大连化学物理研究所
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