中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 大连化学物理研究所
  3. 中国科学院大连化学物理研究所
Triacylglycerol accumulates exclusively outside the chloroplast in short-term nitrogen-deprived Chlamydomonas reinhardtii

文献类型：期刊论文

作者Yang, Miao2,3,4; Meng, Yingying1,2; Chu, Yadong2; Fan, Yan2,4; Cao, Xupeng2; Xue, Song2; Chi, Zhanyou3
刊名BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
出版日期2018-12-01
卷号1863期号:12页码:1478-1487
ISSN号1388-1981
关键词Chlamydomonas Chloroplast Extra-chloroplastidic TAG accumulation Membrane glycerolipids Short-term nitrogen starvation Subcellular glycerolipidomics
DOI10.1016/j.bbalip.2018.09.009
通讯作者Xue, Song(xuesong@dicp.ac.cn)
英文摘要In microalgae, triacylglycerol (TAG) biosynthesis occurs by parallel pathways involving both the chloroplast and endoplasmic reticulum. A better understanding of contribution of each pathway to TAG assembly facilitates enhanced TAG production via rational genetic engineering of microalgae. Here, using a UPLC-MS(/MS) coupled with TLC-GC-based lipidomic platform, the early response of the major glycerolipids to nitrogen stress was analyzed at both the cellular and chloroplastidic levels in the model green alga Chlamydomonas reinhardtii. Subcellular lipidomic analysis demonstrated that TAG was accumulated exclusively outside the chloroplast, and remained unaltered inside the chloroplast after 4 h of nitrogen starvation. This study ascertained the existence of the glycolipid, digalactosyldiacylglycerol (DGDG), outside the chloroplast and the betaine lipid, diacylglycerol-N,N,N-trimethylhomoserine (DGTS), inside the chloroplast. The newly synthesized DGDG and DGTS prominently increased at the extra-chloroplastidic compartments and served as the major precursors for TAG biosynthesis. In particular, DGDG contributed to the extra-chloroplastidic TAG assembly in form of diacylglycerol (DAG) and DGTS in form of acyl groups. The chloroplastidic membrane lipid, monogalactosyldiacylglycerol (MGDG), was proposed to primarily offer DAG for TAG formation outside the chloroplast. This study provides valuable insights into the subcellular glycerolipidomics and unveils the acyl flux into the extra-chloroplastidic TAG in microalgae.
WOS关键词ACID BETA-OXIDATION ; LIPID-METABOLISM ; NANNOCHLOROPSIS-OCEANICA ; MEMBRANE PHOSPHOLIPIDS ; PHOSPHATE DEPRIVATION ; PLASMA-MEMBRANE ; BIOSYNTHESIS ; MICROALGAE ; ACYLTRANSFERASE ; IDENTIFICATION
资助项目National Natural Science Foundation of China[21576253] ; National Natural Science Foundation of China[31470432]
WOS研究方向Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
语种英语
出版者ELSEVIER SCIENCE BV
WOS记录号WOS:000449898800005
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://cas-ir.dicp.ac.cn/handle/321008/166630]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Xue, Song
作者单位1.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China
3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Miao,Meng, Yingying,Chu, Yadong,et al. Triacylglycerol accumulates exclusively outside the chloroplast in short-term nitrogen-deprived Chlamydomonas reinhardtii[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS,2018,1863(12):1478-1487.
APA Yang, Miao.,Meng, Yingying.,Chu, Yadong.,Fan, Yan.,Cao, Xupeng.,...&Chi, Zhanyou.(2018).Triacylglycerol accumulates exclusively outside the chloroplast in short-term nitrogen-deprived Chlamydomonas reinhardtii.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS,1863(12),1478-1487.
MLA Yang, Miao,et al."Triacylglycerol accumulates exclusively outside the chloroplast in short-term nitrogen-deprived Chlamydomonas reinhardtii".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS 1863.12(2018):1478-1487.

入库方式： OAI收割

来源：大连化学物理研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。