Highly hydrophilic carbon nanoparticles: uptake mechanism by mammalian and plant cells
文献类型：期刊论文
| 作者 | Peng, Xiaojun1; Chen, Lijuan1,3,4; Wang, Hongbo5; Li, Xiang5; Nie, Cong5; Liang, Taibo2; Xie, Fuwei5; Liu, Kejian5; Xie, Jianping5 |
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2018 |
| 卷号 | 8期号:61页码:35246-35256 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c8ra06665e |
| 通讯作者 | Xie, Jianping(ztridicp@126.com) |
| 英文摘要 | As one of the carbon based materials, the potential application of carbon nanoparticles (CNPs) has emerged in the promotion of plant growth. However, knowledge on the biological mechanism of how the CNPs interact with plant cells is limited. In this study, nanostructures of CNPs were examined. The particles exhibited particulate morphology and their size distribution was in the range of 18 to 70 nm, with an average size of 30 nm. Hydrophilic groups of COOH and OH were present on the surface of CNPs, and CNPs showed the common feature of graphitic sp(2) hybridization carbons. The CNPs were determined to be biocompatible with these two cell lines, mammalian cells (A549 cells) and plant cells (BY-2 cells). The COOH groups on the surface of CNPs were functionalized via covalent binding with a fluorescent dye for improvement of the fluorescence. The fluorescent carbon nanoparticles (FCNPs) were found to cross the cell membrane and enter cells (A549 cells and BY-2 cells) in an energy-dependent manner. Subsequently, the mechanism of FCNPs interaction with the cell membrane was evaluated in the presence of inhibitors that specifically affect different endocytosis membrane proteins. The FCNPs mainly entered A549 cells through caveolin-mediated endocytosis and macropinocytosis, and clathrin-dependent endocytosis was also involved in the transportation of the FCNPs. Clathrin-independent endocytosis mediated in the internalization of FCNPs in BY-2 cells. The way FCNPs entering cells will provide a fundamental understanding of the influence of CNPs on cell membrane. |
| WOS关键词 | SOLID-STATE NMR ; GRAPHITE OXIDE ; EPITHELIAL-CELLS ; GRAPHENE OXIDE ; ENDOCYTOTIC UPTAKE ; NANOTUBES ; ACID ; FUNCTIONALIZATION ; MACROPHAGES ; PALLADIUM |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000448348600055 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/166770]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Xie, Jianping |
| 作者单位 | 1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Liaoning, Peoples R China 2.CNTC, Zhengzhou Tobacco Res Inst, Key Lab Ecol Environm & Tobacco Qual, 2 Fengyang Rd, Zhengzhou 450001, Henan, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China 4.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China 5.CNTC, Zhengzhou Tobacco Res Inst, Key Lab Tobacco Chem, 2 Fengyang Rd, Zhengzhou 450001, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Peng, Xiaojun,Chen, Lijuan,Wang, Hongbo,et al. Highly hydrophilic carbon nanoparticles: uptake mechanism by mammalian and plant cells[J]. RSC ADVANCES,2018,8(61):35246-35256. |
| APA | Peng, Xiaojun.,Chen, Lijuan.,Wang, Hongbo.,Li, Xiang.,Nie, Cong.,...&Xie, Jianping.(2018).Highly hydrophilic carbon nanoparticles: uptake mechanism by mammalian and plant cells.RSC ADVANCES,8(61),35246-35256. |
| MLA | Peng, Xiaojun,et al."Highly hydrophilic carbon nanoparticles: uptake mechanism by mammalian and plant cells".RSC ADVANCES 8.61(2018):35246-35256. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。