Quality Control of Serum and Plasma by Quantification of (4E, 14Z)-Sphingadienine-C18-1-Phosphate Uncovers Common Preanalytical Errors During Handling of Whole Blood
文献类型：期刊论文
| 作者 | Mendy, Maimuna14; Weigert, Cora4,6; Wang, Xiaolin2; Fritsche, Andreas4,5,6; Peter, Andreas4,5,6; Haering, Hans-Ulrich4,5,6; Lehmann, Rainer4,5,6; Xu, Guowang2; Liu, Xinyu2,3; Hoene, Miriam4 |
| 刊名 | CLINICAL CHEMISTRY
 |
| 出版日期 | 2018-05-01 |
| 卷号 | 64期号:5页码:810-819 |
| ISSN号 | 0009-9147 |
| DOI | 10.1373/clinchem.2017.277905 |
| 通讯作者 | Xu, Guowang(xugw@dicp.ac.cn) ; Lehmann, Rainer(rainer.lehmann@med.uni-tuebingen.de) |
| 英文摘要 | BACKGROUND: Nonadherence to standard operating procedures (SOPs) during handling and processing of whole blood is one of the most frequent causes affecting the quality of serum and plasma. Yet, the quality of blood samples is of the utmost importance for reliable, conclusive research findings, valid diagnostics, and appropriate therapeutic decisions. METHODS: UHPLC-MS-driven nontargeted metabolomics was applied to identify biomarkers that reflected time to processing of blood samples, and a targeted UHPLC-MS analysis was used to quantify and validate these biomarkers. RESULTS: We found that (4E, 14Z)-sphingadienine-C181-phosphate (S1P-d18: 2) was suitable for the reliable assessment of the pronounced changes in the quality of serum and plasma caused by errors in the phase between collection and centrifugation of whole blood samples. We rigorously validated S1P-d18: 2, which included the use of practicality tests on >1400 randomly selected serum and plasma samples that were originally collected during single-and multicenter trials and then stored in 11 biobanks in 3 countries. Neither life-threatening disease states nor strenuous metabolic challenges (i.e., highintensity exercise) affected the concentration of S1P-d18: 2. Cutoff values for sample assessment were defined (plasma, <= 0.085 mu g/mL; serum, <= 0.154 mu g/mL). CONCLUSIONS: Unbiased valid monitoring to check for adherence to SOP-dictated time for processing to plasma or serum and/or time to storage of whole blood at 4 degrees C is now feasible. This novel quality assessment step could enable scientists to uncover common preanalytical errors, allowing for identification of serum and plasma samples that should be excluded from certain investigations. It should also allow control of samples before long-term storage in biobanks. (c) 2018 American Association for Clinical Chemistry |
| WOS关键词 | SPHINGOSINE 1-PHOSPHATE ; UK BIOBANK ; WASTE |
| 资助项目 | National Key Research and Development Program of China[2017YFC0906900] ; National Science Foundation of China[81472374] ; Sino-German Center for Research Promotion - National Science Foundation of China[GZ 753] ; German Research Foundation[LE 1391/1-1] ; German Center for Diabetes Research ; German Federal Ministry of Education and Research[01GI0925] |
| WOS研究方向 | Medical Laboratory Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000448290000010 |
| 出版者 | AMER ASSOC CLINICAL CHEMISTRY |
| 资助机构 | National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Science Foundation of China ; National Science Foundation of China ; Sino-German Center for Research Promotion - National Science Foundation of China ; Sino-German Center for Research Promotion - National Science Foundation of China ; German Research Foundation ; German Research Foundation ; German Center for Diabetes Research ; German Center for Diabetes Research ; German Federal Ministry of Education and Research ; German Federal Ministry of Education and Research ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Science Foundation of China ; National Science Foundation of China ; Sino-German Center for Research Promotion - National Science Foundation of China ; Sino-German Center for Research Promotion - National Science Foundation of China ; German Research Foundation ; German Research Foundation ; German Center for Diabetes Research ; German Center for Diabetes Research ; German Federal Ministry of Education and Research ; German Federal Ministry of Education and Research ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Science Foundation of China ; National Science Foundation of China ; Sino-German Center for Research Promotion - National Science Foundation of China ; Sino-German Center for Research Promotion - National Science Foundation of China ; German Research Foundation ; German Research Foundation ; German Center for Diabetes Research ; German Center for Diabetes Research ; German Federal Ministry of Education and Research ; German Federal Ministry of Education and Research ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Science Foundation of China ; National Science Foundation of China ; Sino-German Center for Research Promotion - National Science Foundation of China ; Sino-German Center for Research Promotion - National Science Foundation of China ; German Research Foundation ; German Research Foundation ; German Center for Diabetes Research ; German Center for Diabetes Research ; German Federal Ministry of Education and Research ; German Federal Ministry of Education and Research |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/166792]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Lehmann, Rainer; Xu, Guowang |
| 作者单位 | 1.Univ Thessaly, Lab Biometry, Volos, Greece 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Univ Hosp Tubingen, Cent Lab, Div Clin Chem & Pathobiochem, Hoppe Seyler Str 3, D-72076 Tubingen, Germany 5.Univ Tubingen, Helmholtz Ctr Munich, Inst Diabet Res & Metab Dis, Clin Chem Lab,German Ctr Diabet Res DZD,Core Faci, Tubingen, Germany 6.German Ctr Diabet Res DZD, Tubingen, Germany 7.Rigshosp, Dept Clin Biochem, Copenhagen, Denmark 8.Rigshosp, Ctr Inflammat & Metab, Copenhagen, Denmark 9.Rigshosp, Ctr Phys Act Res, Dept Infect Dis, Copenhagen, Denmark 10.Rigshosp, Copenhagen Muscle Res Ctr, Copenhagen, Denmark |
| 推荐引用方式 GB/T 7714 | Mendy, Maimuna,Weigert, Cora,Wang, Xiaolin,et al. Quality Control of Serum and Plasma by Quantification of (4E, 14Z)-Sphingadienine-C18-1-Phosphate Uncovers Common Preanalytical Errors During Handling of Whole Blood[J]. CLINICAL CHEMISTRY,2018,64(5):810-819. |
| APA | Mendy, Maimuna.,Weigert, Cora.,Wang, Xiaolin.,Fritsche, Andreas.,Peter, Andreas.,...&Haap, Michael.(2018).Quality Control of Serum and Plasma by Quantification of (4E, 14Z)-Sphingadienine-C18-1-Phosphate Uncovers Common Preanalytical Errors During Handling of Whole Blood.CLINICAL CHEMISTRY,64(5),810-819. |
| MLA | Mendy, Maimuna,et al."Quality Control of Serum and Plasma by Quantification of (4E, 14Z)-Sphingadienine-C18-1-Phosphate Uncovers Common Preanalytical Errors During Handling of Whole Blood".CLINICAL CHEMISTRY 64.5(2018):810-819. |
入库方式： OAI收割
来源：大连化学物理研究所
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