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An Efficient Approach for Selective Enrichment of Histone Modification Readers Using Self-Assembled Multivalent Photoaffinity Peptide Probes

文献类型：期刊论文

作者Zhang, Kai3; Zhai, Guijin3; Dong, Hanyang3; Guo, Zhenchang3; Feng, Wei4; Jin, Jin1; Zhang, Tao4; Chen, Cong3; Chen, Pu3; Tian, Shanshan3
刊名ANALYTICAL CHEMISTRY
出版日期2018-10-02
卷号90期号:19页码:11385-11392
ISSN号0003-2700
DOI10.1021/acs.analchem.8b02342
通讯作者Zhang, Kai(kzhang@tmu.edu.cn)
英文摘要Histone post-translational modifications (HPTMs) provide signaling platforms to recruit proteins or protein complexes (e.g., transcription factors, the so-called "readers" of the histone code), changing DNA accessibility in the regulation of gene expression. Thus, it is an essential task to identify HPTM readers for understanding of epigenetic regulation. Herein we designed and prepared a novel HPTM probe based on self-assembled multivalent photo-cross-linking technique for selective enrichment and identification of HPTM readers. By use of trimethylation of histone H3 lysine 4, we showcased that the functionalized HPTM probe was able to capture its reader with high enrichment efficiency and remarkable specificity even in a complex environment. Notably, this approach was readily applicable for exploring crosstalk among multiple HPTMs. Combining the probes with a mass spectrometry-based proteomic approach, our approach reached a fairly high coverage of known H3K4me3 readers. We further demonstrated that the HPTM probes can enrich a new type of HPTM readers and uncovered several novel putative binders of crotonylation of histone H3 lysine 9, expanding the repertoire of readers for this epigenetic mark. More broadly, our work provides a general strategy for rapid and robust interrogating HPTM readers and will be of great importance to elucidate epigenetic mechanism in regulating gene activity.
WOS关键词MODIFIED GOLD NANOPARTICLES ; PHD FINGER ; YEATS DOMAIN ; PROTEINS ; CROTONYLATION ; TRANSCRIPTION ; IDENTIFICATION ; ACETYLATION ; PROTEOMICS ; DNA
资助项目National Natural Science Foundation of China[21874100] ; National Natural Science Foundation of China[21275077] ; National Natural Science Foundation of China[21705067] ; National Basic Research Program of China[2016YFC0903000] ; Talent Excellence Program from Tianjin Medical University
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000446542600032
出版者AMER CHEMICAL SOC
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Talent Excellence Program from Tianjin Medical University ; Talent Excellence Program from Tianjin Medical University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Talent Excellence Program from Tianjin Medical University ; Talent Excellence Program from Tianjin Medical University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Talent Excellence Program from Tianjin Medical University ; Talent Excellence Program from Tianjin Medical University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Talent Excellence Program from Tianjin Medical University ; Talent Excellence Program from Tianjin Medical University
源URL[http://cas-ir.dicp.ac.cn/handle/321008/166812]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Zhang, Kai
作者单位1.Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China
3.Tianjin Med Univ, Canc Inst & Hosp,Tianjin Key Lab Med Epigenet,Col, Key Lab Breast Canc Prevent & Treatment,Minist Ed, Dept Biochem & Mol Biol,Key Lab Immune Microenvir, Tianjin 300070, Peoples R China
4.Tianjin Med Univ, Sch Biomed Engn, Tianjin 300070, Peoples R China
5.Univ Freiburg, Inst Biochem & Mol Biol, Stefan Meier Str 17, D-79104 Freiburg, Germany
推荐引用方式
GB/T 7714		 Zhang, Kai,Zhai, Guijin,Dong, Hanyang,et al. An Efficient Approach for Selective Enrichment of Histone Modification Readers Using Self-Assembled Multivalent Photoaffinity Peptide Probes[J]. ANALYTICAL CHEMISTRY,2018,90(19):11385-11392.
APA Zhang, Kai.,Zhai, Guijin.,Dong, Hanyang.,Guo, Zhenchang.,Feng, Wei.,...&Zhang, Yukui.(2018).An Efficient Approach for Selective Enrichment of Histone Modification Readers Using Self-Assembled Multivalent Photoaffinity Peptide Probes.ANALYTICAL CHEMISTRY,90(19),11385-11392.
MLA Zhang, Kai,et al."An Efficient Approach for Selective Enrichment of Histone Modification Readers Using Self-Assembled Multivalent Photoaffinity Peptide Probes".ANALYTICAL CHEMISTRY 90.19(2018):11385-11392.

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来源：大连化学物理研究所

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