Accurate quantification of astaxanthin from Haematococcus pluvialis using DMSO extraction and lipase-catalyzed hydrolysis pretreatment
文献类型：期刊论文
| 作者 | Liu, Jiao3,4; Xue, Song3; Wang, Shuyan2,3; Meng, Yingying1,3; Cao, Xupeng3 |
| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
 |
| 出版日期 | 2018-11-01 |
| 卷号 | 35页码:427-431 |
| 关键词 | Quantification of astaxanthin Haematococcus pluvialis Dimethyl sulfoxide extraction Cholesterol esterase hydrolysis |
| ISSN号 | 2211-9264 |
| DOI | 10.1016/j.algal.2018.08.029 |
| 通讯作者 | Xue, Song(xuesong@dicp.ac.cn) |
| 英文摘要 | Haematococcus pluvialis accumulates high contents of astaxanthin and is considered as an important source of natural astaxanthin production. Fast and accurate quantification of astaxanthin is needed to evaluate the quality of microalgal sources for astaxanthin extraction and to monitor the H. pluvialis cultivation process. In the present study, a less-invasive method for astaxanthin accurate quantification was developed. Dimethyl sulfoxide (DMSO) was used as an extraction solvent to improve the membrane permeability of H. pluvialis. Cholesterol esterase was used to hydrolyze astaxanthin esters via incubation at 36 degrees C for 30 min. The detection of astaxanthin after hydrolysis was achieved by high performance liquid chromatography (HPLC) within 10 min using a C18 column. The astaxanthin content measurements after enzymatic pretreatment were 1.5-1.7 times higher than that measured after saponified pretreatment. DMSO solvent extraction combined with lipase-catalyzed hydrolysis followed by HPLC detection is proposed for the accurate quantification of astaxanthin in H. pluvialis. |
| WOS关键词 | MICROALGAE ; ESTERS ; ACCUMULATION |
| 资助项目 | Educational Department of Inner Mongolia[NJZY168] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000447187700046 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia ; Educational Department of Inner Mongolia |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/166890]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Xue, Song |
| 作者单位 | 1.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China 2.Inner Mongolia Univ Nationlities, Chem & Chem Engn Coll, Tongliao 028000, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China 4.Qilu Univ Technol, Dept Bioengn, Jinan 250353, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jiao,Xue, Song,Wang, Shuyan,et al. Accurate quantification of astaxanthin from Haematococcus pluvialis using DMSO extraction and lipase-catalyzed hydrolysis pretreatment[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2018,35:427-431. |
| APA | Liu, Jiao,Xue, Song,Wang, Shuyan,Meng, Yingying,&Cao, Xupeng.(2018).Accurate quantification of astaxanthin from Haematococcus pluvialis using DMSO extraction and lipase-catalyzed hydrolysis pretreatment.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,35,427-431. |
| MLA | Liu, Jiao,et al."Accurate quantification of astaxanthin from Haematococcus pluvialis using DMSO extraction and lipase-catalyzed hydrolysis pretreatment".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 35(2018):427-431. |
入库方式： OAI收割
来源：大连化学物理研究所
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