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Serum or plasma, what is the difference? Investigations to facilitate the sample material selection decision making process for metabolomics studies and beyond

文献类型：期刊论文

作者Liu, Xinyu1; Hoene, Miriam2; Wang, Xiaolin1; Yin, Peiyuan1; Xu, Guowang1; Haering, Hans-Ulrich2,3,4; Lehmann, Rainer2,3,4
刊名ANALYTICA CHIMICA ACTA
出版日期2018-12-11
卷号1037页码:293-300
关键词Plasma Serum Metabolic profiling Sample material selection Metabolomics
ISSN号0003-2670
DOI10.1016/j.aca.2018.03.009
通讯作者Xu, Guowang(xugw@dicp.ac.cn) ; Lehmann, Rainer(rainer.lehmann@med.uni-tuebingen.de)
英文摘要In analytical chemistry serum as well as plasma are recommended as sample material of choice. However, blood processing for the generation of serum or plasma is rather different. Whether plasma or serum is the preferable sample material is still controversial discussed. We performed in paired samples three UHPLC-mass spectrometry-driven metabolomics studies. In study 1 metabolite profiles of serum vs plasma were compared. 46% out of 216 identified metabolites showed significant different levels (paired Wilcoxon signed-rank test, p < 0.05, FDR <0.01) with only three metabolites (methionine, C2:0- and C3:0-carnitine) showing lower levels in serum. In study 2 comparison of three different serum blood collection tubes revealed that coagulation and associated processes distinctly alter metabolite levels depending on the tube-specific clotting process. Most pronounced differences were found for the dipeptide phenylalanine-phenylalanine (highest levels in silicate containing serum blood collection tubes). In study 3 possible adverse effects of platelets, which still remain in standard plasma even after correct processing, were investigated. No differences in a pattern of 216 metabolites were detected in the comparison of standard and platelet-free plasma (PFP). Our results give novel insights in fundamental differences between serum and plasma, thereby providing valuable information for analytical chemists for decision making to either use serum or plasma before starting complex and time-consuming analytical investigations. (C) 2018 Elsevier B.V. All rights reserved.
WOS关键词MASS-SPECTROMETRY ; HUMAN BLOOD ; GAS-CHROMATOGRAPHY ; COLLECTION
资助项目National Key Research and Development Program of China[2017YFC0906900] ; Sino-German Center for Research Promotion (DFG)[GZ 753] ; Sino-German Center for Research Promotion (NSFC)[LE 1391/1-1] ; German Federal Ministry of Education and Research (BMBF)[01GI0925] ; key foundation and creative research group project (NSFC)[21435006] ; key foundation and creative research group project (NSFC)[21321064]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000446225000030
出版者ELSEVIER SCIENCE BV
资助机构National Key Research and Development Program of China ; National Key Research and Development Program of China ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; German Federal Ministry of Education and Research (BMBF) ; German Federal Ministry of Education and Research (BMBF) ; key foundation and creative research group project (NSFC) ; key foundation and creative research group project (NSFC) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; German Federal Ministry of Education and Research (BMBF) ; German Federal Ministry of Education and Research (BMBF) ; key foundation and creative research group project (NSFC) ; key foundation and creative research group project (NSFC) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; German Federal Ministry of Education and Research (BMBF) ; German Federal Ministry of Education and Research (BMBF) ; key foundation and creative research group project (NSFC) ; key foundation and creative research group project (NSFC) ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; German Federal Ministry of Education and Research (BMBF) ; German Federal Ministry of Education and Research (BMBF) ; key foundation and creative research group project (NSFC) ; key foundation and creative research group project (NSFC)
源URL[http://cas-ir.dicp.ac.cn/handle/321008/166914]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Xu, Guowang; Lehmann, Rainer
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Hosp Tubingen, Cent Lab, Div Clin Chem & Pathobiochem, Hoppe Seyler Str 3, D-72076 Tubingen, Germany
3.German Ctr Diabet Res DZD, Neuherberg, Germany
4.Univ Tubingen, Helmholtz Ctr Munich, Inst Diabet Res & Metab Dis, Core Facil DZD Clin Chem Lab, Tubingen, Germany
推荐引用方式
GB/T 7714		 Liu, Xinyu,Hoene, Miriam,Wang, Xiaolin,et al. Serum or plasma, what is the difference? Investigations to facilitate the sample material selection decision making process for metabolomics studies and beyond[J]. ANALYTICA CHIMICA ACTA,2018,1037:293-300.
APA Liu, Xinyu.,Hoene, Miriam.,Wang, Xiaolin.,Yin, Peiyuan.,Xu, Guowang.,...&Lehmann, Rainer.(2018).Serum or plasma, what is the difference? Investigations to facilitate the sample material selection decision making process for metabolomics studies and beyond.ANALYTICA CHIMICA ACTA,1037,293-300.
MLA Liu, Xinyu,et al."Serum or plasma, what is the difference? Investigations to facilitate the sample material selection decision making process for metabolomics studies and beyond".ANALYTICA CHIMICA ACTA 1037(2018):293-300.

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来源：大连化学物理研究所

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