Proteomics investigation of the changes in serum proteins after high- and low-flux hemodialysis
文献类型：期刊论文
| 作者 | Zhao, Jiuyang1; Zhu, Hong3; Liu, Jianhui2; Zhang, Lihua2; Han, Shuai3; Yang, Kaiguang2 |
| 刊名 | RENAL FAILURE
 |
| 出版日期 | 2018-10-03 |
| 卷号 | 40期号:1页码:506-513 |
| 关键词 | Proteomics serum proteins hemodialysis dialyzer |
| ISSN号 | 0886-022X |
| DOI | 10.1080/0886022X.2018.1491406 |
| 通讯作者 | Zhang, Lihua(lihuazhang@dicp.ac.cn) ; Zhao, Jiuyang(zhaojiuy9@163.com) |
| 英文摘要 | Purpose: This study aimed to use proteomics methods to investigate the changes in serum protein levels after high- and low-flux hemodialysis (HD). Methods: Before and after HD, serum samples were obtained from two selected patients who were treated with a Polyflux 140H high-flux dialyzer and a Polyflux 14L low-flux dialyzer during two continuous therapy sessions. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was performed to identify the proteins. Results: A total of 212 and 203 serum proteins were identified after high-flux and low-flux HD, respectively. After high-flux HD, 21 proteins increased, and 132 proteins decreased. After low-flux HD, 87 proteins increased, and 45 proteins decreased. High-flux HD led to a significantly greater reduction in protein levels than low-flux HD (0.73 +/- 0.13 vs. 0.84 +/- 0.18, p = .00). Among the increased and decreased proteins, the isoelectric point (pI) values mainly ranged from 5 to 7, and the molecular weights (Mws) were mostly smaller than 30 kDa. The serum proteins showed no difference in pI or Mw for high- and low-flux HD. Gene ontology (GO) analysis showed that the detected proteins were related to immune system processes and complement activation. Conclusions: Serum protein levels differentially changed after high- and low-flux HD. Long-term effects should be observed in future studies. |
| WOS关键词 | CHRONIC KIDNEY-DISEASE ; TANDEM MASS-SPECTROMETRY ; D-BINDING PROTEIN ; ONLINE HEMODIAFILTRATION ; DIALYSIS MEMBRANES ; NEPHROTIC SYNDROME ; OXIDATIVE STRESS ; STABLE-ISOTOPE ; ADSORPTION ; HDL |
| WOS研究方向 | Urology & Nephrology |
| 语种 | 英语 |
| WOS记录号 | WOS:000446358100001 |
| 出版者 | TAYLOR & FRANCIS LTD |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/166966]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhao, Jiuyang; Zhang, Lihua |
| 作者单位 | 1.Dalian Med Univ, Affiliated Hosp 2, Dept Nephrit Med, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China 3.Dalian Univ, Affiliated Zhongshan Hosp, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Jiuyang,Zhu, Hong,Liu, Jianhui,et al. Proteomics investigation of the changes in serum proteins after high- and low-flux hemodialysis[J]. RENAL FAILURE,2018,40(1):506-513. |
| APA | Zhao, Jiuyang,Zhu, Hong,Liu, Jianhui,Zhang, Lihua,Han, Shuai,&Yang, Kaiguang.(2018).Proteomics investigation of the changes in serum proteins after high- and low-flux hemodialysis.RENAL FAILURE,40(1),506-513. |
| MLA | Zhao, Jiuyang,et al."Proteomics investigation of the changes in serum proteins after high- and low-flux hemodialysis".RENAL FAILURE 40.1(2018):506-513. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。