Macro-mesoporous organosilica monoliths with bridged-ethylene and terminal-vinyl: High-density click functionalization for chromatographic separation
文献类型：期刊论文
| 作者 | Liang, Zhen2; Zhang, Yukui2; Wu, Ci1,2; Liang, Yu2; Zhu, Xudong2; Zhao, Qun2; Fang, Fei2; Zhang, Xiaodan2; Zhang, Lihua2 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2018-12-14 |
| 卷号 | 1038页码:198-205 |
| 关键词 | Hybrid monolith Mesoporosity Ethylene-bridged organosilane Peptide separation Proteomics |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2018.07.004 |
| 通讯作者 | Zhang, Lihua(lihuazhang@dicp.ac.cn) |
| 英文摘要 | A novel kind of macro-mesoporous organosilica monolith, with not only bridged-ethylene groups incorporated into the skeleton but also terminal-vinyl groups protruded from the pore-wall, was prepared so that high-loaded double bonds were achieved. Via highly efficient "thiol-ene" click reaction of such high-loaded double bonds, the surface coverage of C18 groups on monolith could be 5.54 mu mol m(-2), significantly larger than that of the reported separation materials, beneficial to improvement of separation resolution, especially for peptide separation. The separation performance was evaluated using alkylbenzenes and standard peptides. Furthermore, the tryptic digests of complex sample was successfully analyzed. Because of high separation resolution of our prepared hybrid monolith, the peak capacity for 6-h gradient was achieved as 482. Coupling to LTQ Orbitrap Velos Mass Spectrometry, 22523 tryptic peptides from 4423 proteins were identified from the HeLa cells, more than that using the other long-gradient separation by the same system reported, showing great promising of such monolith for large-scale in-depth proteomic analysis. (C) 2018 Elsevier B.V. All rights reserved. |
| WOS关键词 | PHASE LIQUID-CHROMATOGRAPHY ; STATIONARY-PHASE ; ADSORPTION MECHANISM ; SURFACE COVERAGE ; SILICA MONOLITHS ; ORGANIC GROUPS ; COLUMNS ; CHEMISTRY ; POLYMER |
| 资助项目 | National Key R&D program of China[2017YFA0505003] ; National Key R&D program of China[2016YFA0501401] ; National Natural Science Foundation of China[21575139] ; National Natural Science Foundation of China[21705065] ; National Natural Science Foundation of China[21235005] ; National Natural Science Foundation of China[91543201] ; National Basic Research Program of China[2013CB911202] ; National key scientific instrument and equipment development projects[2012YQ120044-8] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000445895500024 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | National Key R&D program of China ; National Key R&D program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National key scientific instrument and equipment development projects ; National key scientific instrument and equipment development projects ; National Key R&D program of China ; National Key R&D program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National key scientific instrument and equipment development projects ; National key scientific instrument and equipment development projects ; National Key R&D program of China ; National Key R&D program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National key scientific instrument and equipment development projects ; National key scientific instrument and equipment development projects ; National Key R&D program of China ; National Key R&D program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National key scientific instrument and equipment development projects ; National key scientific instrument and equipment development projects |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/166982]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Lihua |
| 作者单位 | 1.Inspect & Quarantine Tech Ctr Dalian Entry Exit I, Dalian 116100, Peoples R China 2.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Zhen,Zhang, Yukui,Wu, Ci,et al. Macro-mesoporous organosilica monoliths with bridged-ethylene and terminal-vinyl: High-density click functionalization for chromatographic separation[J]. ANALYTICA CHIMICA ACTA,2018,1038:198-205. |
| APA | Liang, Zhen.,Zhang, Yukui.,Wu, Ci.,Liang, Yu.,Zhu, Xudong.,...&Zhang, Lihua.(2018).Macro-mesoporous organosilica monoliths with bridged-ethylene and terminal-vinyl: High-density click functionalization for chromatographic separation.ANALYTICA CHIMICA ACTA,1038,198-205. |
| MLA | Liang, Zhen,et al."Macro-mesoporous organosilica monoliths with bridged-ethylene and terminal-vinyl: High-density click functionalization for chromatographic separation".ANALYTICA CHIMICA ACTA 1038(2018):198-205. |
入库方式： OAI收割
来源：大连化学物理研究所
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