Rational design of sequestered DNAzyme beacons to enable flexible control of catalytic activities
文献类型：期刊论文
| 作者 | Dai, Danling2; Liu, Feng1; Tang, Wei2; Cheng, Mengxi2; Xiong, Zhonghua2 |
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2018 |
| 卷号 | 8期号:51页码:29338-29343 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c8ra05757e |
| 通讯作者 | Tang, Wei(weitang@caep.cn) |
| 英文摘要 | DNAzymes as functional units play increasingly important roles for DNA nanotechnology, and fine control of the catalytic activities of DNAzymes is a crucial element in the design and construction of functional and dynamic devices. So far, attempts to control cleavage kinetics can be mainly achieved through varying the concentrations of the specific metal ions. Here we present a facile sequestered DNAzyme beacon strategy based on precisely blocking the catalytic core of the DNAzyme, which can flexibly regulate the DNAzyme cleavage kinetics without changing the concentrations of metal ions. This strategy can be extended to couple with a large number of other RNA-cleaving DNAzymes and was successfully applied in designing a dual stem-loop structure probe for arbitrary sequence biosensing, which provides the possibility of scaling up versatile and dynamic DNA devices that use DNAzymes as functional modules. |
| WOS关键词 | IN-VITRO SELECTION ; NUCLEIC-ACID ; COMPUTING CIRCUITS ; DEPENDENT DNAZYMES ; LOGIC GATES ; DNA ENZYME ; METAL-IONS ; DEOXYRIBOZYME ; NANOPARTICLES ; SENSITIVITY |
| 资助项目 | National Natural Science Foundation of China[21605134] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000444700100053 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167004]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Tang, Wei |
| 作者单位 | 1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.China Acad Engn Phys, Inst Mat, Mianyang 621700, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Danling,Liu, Feng,Tang, Wei,et al. Rational design of sequestered DNAzyme beacons to enable flexible control of catalytic activities[J]. RSC ADVANCES,2018,8(51):29338-29343. |
| APA | Dai, Danling,Liu, Feng,Tang, Wei,Cheng, Mengxi,&Xiong, Zhonghua.(2018).Rational design of sequestered DNAzyme beacons to enable flexible control of catalytic activities.RSC ADVANCES,8(51),29338-29343. |
| MLA | Dai, Danling,et al."Rational design of sequestered DNAzyme beacons to enable flexible control of catalytic activities".RSC ADVANCES 8.51(2018):29338-29343. |
入库方式： OAI收割
来源：大连化学物理研究所
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